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This experiment is to find out how different temperatures affect the rate at how an enzymes activity differs , using catalase and hydrogen peroxide.

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Introduction

Catalase and Hydrogen Peroxide Experiment Introduction: This experiment is to find out how different temperatures affect the rate at how an enzymes activity differs. The enzymes that we are going to use are called catalyses from a plant, the plant we are going to use s celery. Inside the cells is hydrogen peroxide. This is a bi-product of respiration along with water and oxygen; we are going to measure the oxygen. Hydrogen peroxide is H2o2 it is a bleach and corrosive substance too much of it in the body can cause death. It is broken up by catalyses to water (h2o) and Oxygen (o2) [image002.gif] H2o2 2H20 + O2 Catalyses is the perfect shape to fit on Hydrogen peroxide The Enzyme locks onto the substrate The Enzyme and substrate comply The enzyme brakes up the substrate A Catalyses is a produced by living cells to brake up hydrogen peroxide it is an enzyme, it brakes Hydrogen peroxide into water and oxygen. It is important so that Hydrogen peroxide does not kill the cells it is found in any living cells. ...read more.

Middle

4. We did this with 20�C, 30�C, 40�C, 50�C and 60�C. using the ice and the boiling water to produce the temperature Trial Experiment I did a trail experiment before I did my real experiment. I did this because than I would know what to do when it come to doing the experiment for real, as it is important to do this experiment in one lesson as the hydrogen peroxide can change after a period of time the aspects that can affect the hydrogen peroxide are: Ultra violet light Temperature I used for my trial experiment: 5ml of celery 30ml of hydrogen peroxide 50ml measuring cylinder I have changed my experiment I will now use: 2ml of celery 20ml of hydrogen peroxide 20ml measuring cylinder I have changed these because the celery and the hydrogen peroxide were both to much and the reaction bubbled over the boiling tube. I have changed the measuring cylinder because I do not need a 50ml one and a 20ml is a lot more accurate. ...read more.

Conclusion

on the other side of the partition than the catalyses this way the reaction doesn't start till the stopper is on and the cylinder is tipped so the Hydrogen peroxide and the catalyses react. [image009.gif] [image010.gif] Also I could use a computer to produce the measuring of the gas as computers are more accurate and can count the gas at the millisecond it hits the time, instead of some one using human eye as this is not accurate. Also I could change what I measure, I could measure instead of the gas produced I could measure the weight of the experiment and take times to see the difference in weight. If I was to do this experiment again would also use the equipment changes listed below and I would make sure all the Hydrogen peroxide I use each time I do the experiment are the same by testing them for there Ph. I would do more experiment with a wider range of temperatures so that my experiment would have more accurate average results. This would also make my graph show more anonymous results. ...read more.

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