• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

This experiment is to find out how different temperatures affect the rate at how an enzymes activity differs , using catalase and hydrogen peroxide.

Extracts from this document...


Catalase and Hydrogen Peroxide Experiment Introduction: This experiment is to find out how different temperatures affect the rate at how an enzymes activity differs. The enzymes that we are going to use are called catalyses from a plant, the plant we are going to use s celery. Inside the cells is hydrogen peroxide. This is a bi-product of respiration along with water and oxygen; we are going to measure the oxygen. Hydrogen peroxide is H2o2 it is a bleach and corrosive substance too much of it in the body can cause death. It is broken up by catalyses to water (h2o) and Oxygen (o2) [image002.gif] H2o2 2H20 + O2 Catalyses is the perfect shape to fit on Hydrogen peroxide The Enzyme locks onto the substrate The Enzyme and substrate comply The enzyme brakes up the substrate A Catalyses is a produced by living cells to brake up hydrogen peroxide it is an enzyme, it brakes Hydrogen peroxide into water and oxygen. It is important so that Hydrogen peroxide does not kill the cells it is found in any living cells. ...read more.


4. We did this with 20�C, 30�C, 40�C, 50�C and 60�C. using the ice and the boiling water to produce the temperature Trial Experiment I did a trail experiment before I did my real experiment. I did this because than I would know what to do when it come to doing the experiment for real, as it is important to do this experiment in one lesson as the hydrogen peroxide can change after a period of time the aspects that can affect the hydrogen peroxide are: Ultra violet light Temperature I used for my trial experiment: 5ml of celery 30ml of hydrogen peroxide 50ml measuring cylinder I have changed my experiment I will now use: 2ml of celery 20ml of hydrogen peroxide 20ml measuring cylinder I have changed these because the celery and the hydrogen peroxide were both to much and the reaction bubbled over the boiling tube. I have changed the measuring cylinder because I do not need a 50ml one and a 20ml is a lot more accurate. ...read more.


on the other side of the partition than the catalyses this way the reaction doesn't start till the stopper is on and the cylinder is tipped so the Hydrogen peroxide and the catalyses react. [image009.gif] [image010.gif] Also I could use a computer to produce the measuring of the gas as computers are more accurate and can count the gas at the millisecond it hits the time, instead of some one using human eye as this is not accurate. Also I could change what I measure, I could measure instead of the gas produced I could measure the weight of the experiment and take times to see the difference in weight. If I was to do this experiment again would also use the equipment changes listed below and I would make sure all the Hydrogen peroxide I use each time I do the experiment are the same by testing them for there Ph. I would do more experiment with a wider range of temperatures so that my experiment would have more accurate average results. This would also make my graph show more anonymous results. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Marked by a teacher

    Find out if enzymes work faster or slower at different temperatures.

    5 star(s)

    the substrate has less energy and will not be travelling as fast as it would be in higher temperatures. Overall, lowering the temperature reduces energy of both the enzyme and the substrate, meaning that they are moving slower and will have fewer collisions.

  2. To Investigate how temperature affects the rate at which catalase catalyses hydrogen peroxide.

    The balance has two decimal places, that means that the yeast can be measured out to an accurate 2gs. The more accurate the amount of yeast used, the more accurate the percentage of the yeast solution will be, and the more controlled the investigation.

  1. Investigate how concentration of the enzyme catalase in celery tissue alters the rate of ...

    I have only displayed the results table for the communal, agreed method we used. This diagram below shows the apparatus in its correct format. N.B: The burette is perpendicular to the table. Fig. 5.1 Implementation and Interpretation of the Results of the Hydrogen Peroxide - Catalase reaction.

  2. To investigate the rate at which hydrogen peroxide is broken down by the enzyme ...

    As you can see, the rate of the reaction and the volume of oxygen collected increases as the amount of celery extract increases. This shows what the effect of the enzyme really does. Because I have found these graphs as examples they do not show the same quantities as I intend to use.

  1. An experiment to find of the isotonic point of root vegetables cells in contents ...

    water content in the root vegetable cylinder will be different as its solute potential will be different. Due to this a different root vegetable will have a different isotonic point and therefore my results will have a low level of precision and reliability.

  2. Reaction of Catalase and Hydrogen Peroxide

    Thus the lower the temperature, the lower the kinetic energy of the system and likewise the higher the temperature the greater the kinetic energy of the system. If the kinetic energy of the molecules is low than the rate of reaction will be low.

  1. The effect of Copper Sulphate concentration on Catalase activity on Hydrogen Peroxide.

    In the event that they break or are dropped, they would scatter an enormous quantity of glass pieces and splinters, if they break when containing one of the harmful solutions in them they would also scatter the solution everywhere turning the working station into a high-risk area It has to

  2. This is an experiment to show how different concentration of celery tissue enzyme, catalase ...

    Cofactors, Activators/coenzymes: - Some enzymes, which influence the reaction, are non-protein. They are attached to the enzymes and are called a prosthetic group. Inorganic cofactors are called activators. They Can attach them selves to the active site to make their shape more efficient.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work