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To conduct an investigation to compare the osmotic behaviour of potato and Chinese radish chips in varying concentrations of sucrose solution.

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Introduction

Oliver Carson, RIAC GCSE Investigation Experiment: To conduct an investigation to compare the osmotic behaviour of potato and Chinese radish chips in varying concentrations of sucrose solution. Planning Experimental Procedures (P) Background Information: Osmosis is the movement of water from a high water potential to a low water potential through a selectively permeable membrane. This shall be important to remember throughout this practical and write up, as this is what the foundations of the experiment are based on. Basic Diagram of Osmosis Apparatus: Test tube rack (that is able to hold 14 test tubes), scalpel, forceps, syringe, tile, 14 test tubes, tissue paper, 1.0 molar sucrose solution, water, potato chips and Chinese radish chips. Predictions: I predict that when the concentration of sucrose is low the chips will gain mass and as the concentration of sucrose solution increases the chips will lose mass. This is because when the concentration of sucrose is low there will be a higher concentration of water outside the chips then in them, so water will move into the chips and when the concentration of sucrose is high, there will be a higher concentration of water in the chip, and water would move out of it. This brings me to the conclusion that in; water they will gain between 15- 20%, 0.1 they will gain between 10-15%, 0.2 they will gain between 5-10%, 0.3 they will gain between 1-5%, 0.4 they will lose between 1-5%, 0.5 they will lose between 5-10% and 0.6 they will lose between 10-20%. ...read more.

Middle

1.22 2.67 1.30 2.80 +0.08 +0.13 6.6 4.9 5.8 E-0.4 1.17 2.87 1.15 2.85 -0.02 -0.02 -1.7 -0.7 -1.2 F-0.5 1.10 2.97 1.02 2.66 -0.08 -0.31 -7.3 -10.4 -8.9 G-0.6 1.12 2.65 0.95 2.12 -0.17 -0.53 -15.2 -20.0 -17.6 Radish Tube Mass at Start/g Mass After/g Change/g % Change Av. % Change - 2 cm 5 cm 2 cm 5 cm 2 cm 5 cm 2 cm 5 cm - A-Water 0.95 2.58 1.17 2.96 +0.22 +0.38 23.2 14.7 19.0 B-0.1 1.10 2.61 1.21 2.86 +0.11 +0.25 10.0 9.6 9.8 C-0.2 1.02 2.39 1.11 2.50 +0.09 +0.11 8.8 4.6 6.7 D-0.3 1.09 2.56 1.12 2.63 +0.03 +0.07 2.8 2.7 2.8 E-0.4 1.03 2.71 1.00 2.63 -0.03 -0.08 -2.9 -7.8 -5.4 F-0.5 1.07 2.70 0.98 2.48 -0.09 -0.22 -8.4 -8.1 -8.3 G-0.6 1.11 2.32 0.99 2.11 -0.12 -0.21 -10.8 -9.1 -10.0 Calculations: % Change = Change / Mass at start * 100 e.g.% Change of Potato Tube A (2cm) = 0.20/1.27 * 100 = 15.74 = 15.7 Av. % Change = [Change (2cm) + % Change (5cm)] / 2 e.g. Av. % Change of Potato Tube A = [15.7 + 18.1] / 2 = 16.9 Analysing Evidence and Drawing Conclusions (A) Analysis: When the concentration of sucrose was low the chips gained mass and as the concentration of sucrose increased more mass was lost. In all of the cases, whether the chip gained or lost mass the water moved in or out of the cells by osmosis. ...read more.

Conclusion

After thinking about all the areas that I could have made mistakes and been inaccurate, I think I am lucky not to have any major anomalous results, but the percentage change in the potato chip that was in 0.3 molar sucrose solution does look slightly adrift from the line of best fit. This could be due to any of the reasons shown above and was most likely a mistake with the mixing of the solution with the syringes. The rest do look about right and were pretty much what I expected. If I were to do this experiment again, I would try and seek new ways to make it as accurate a possible; firstly I think I would use four chips for each of the solutions and take an average out of the results, which would greatly improve the accuracy. Also instead of wasting lots of time measuring out the solutions in the syringes, I would use graduated pipettes instead; the measurements on them are far more accurate and would definitely make the concentrations more accurate. Finally, this is not necessary but I believe it would be very interesting to instead of just drying the chips for 10 seconds, to dry them for 10 seconds then weigh them, then dry them for 30 seconds then weigh them and finally dry them for 1 minute and weigh them and find out the percentage difference, which I suspect to be just a couple of percent at most. But I believe my evidence as a whole to be very reliable, except for maybe the potato chip in 0.3 molar sucrose solution, and it is definitely sufficient enough to support me with a firm and correct conclusion. ...read more.

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