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To determine if temperature would, affect the respiration rate of immobilised and non-immobilised yeast cells.

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Introduction

To determine if temperature would, affect the respiration rate of immobilised and non-immobilised yeast cells. Martha Orotayo Prediction: That the optimum rate of immobilised yeast cells would be greater than that of non-immobilised yeast cells due to changes in the temperature. Hypothesis: As I am investigating the effect of temperature upon the respiration rate of immobilised yeast cells its essential to know any relevant background information on yeast cells. Immobilisation is termed as the act or process whereby you prevent a thing from moving. In terms of speaking of immobilised yeast c ells it would men that these cells would be unable to move about. Also the movement in space of an immobilised enzyme is restricted either completely or is limited to a small region. Yeast is composed of proteins and celluloses (much more celluloses involved) and also has a tough outer shell made up of protein and cellulose. Another name for yeast cells is Saccharomyces cerevisiae (meaning a mould that ferments in cereal the sugar to make alcohol and carbon dioxide) which has come to be an important model into the study of Eukaryotic cell's mode of life. ...read more.

Middle

7. Then place this beaker underneath the syringe. 8. Allow beads to stand for 20minutes in the calcium chloride solution. 9. Set up water bath to temperature of 20�C. 10. Once beads are well formed then strain with a tea strainer to separate the beads from the Calcium Chloride solution. 11. Measure 200cm� of 3% sucrose solution into a 250cm� conical flask. 12. Set up the water trough with correct apparatus. 13. Place the conical flask into the water bath. 14. Measure the amount of gas produced. 15. Repeat steps 11-14 for different temperatures. Result table Temperature Immobilised yeast cell (cm�) Non-immobilised yeast cell (cm�) 20 �C 1.1 2.1 25 �C 1.5 1.6 30 �C 3.0 4.3 35 �C 4.0 4.1 40 �C 8.6 1.1 45 �C 10.5 6.6 50 �C 11.6 14.6 Conclusion In conclusion, it appears that the immobilised yeast cells were most effective for this particular experiment because they appear to respire the most within the given time. Also it would appear that the optimum temperature for the immobilised yeast cell was 50�C as the gas collected at it was highest at 11.6cm�. While non-immobilised yeast cell had an optimum temperature of also 50�C but the amount of gas collected was higher with 14.6cm�. ...read more.

Conclusion

I had to use an electrical apparatus (i.e. the water bath) and this carries the potential hazard of giving an electric shock which may be fatal following contact with live components. I had to put into consideration not to tamper with electrical equipment to attempt repairs and to previously check that it was unsafe in any manner. I also checked the re-test dates on it, kept water away from mains electrical sockets. The shards of the broken conical flask were disposed of immediately as they could have caused severe physical injuries. Broken glassware is never cleaned up by hand. Instead dustpans and brushes were used to collect the broken glass and it is disposed of appropriately. I also made sure not to use any damaged glassware. The sucrose solution is considered to be toxic to the central nervous system can induce confusion, fatigue, irritability, and other behavioural changes. I organised my work around my work top to reduce exposure to hazards. Non-latex gloves would be worn during the experiment were I to have any broken skin or rashes on my hands were present during the experiment. Hands were washed after viable materials are handled and before leaving the laboratory and at the conclusion of the experiment. Hands are washed following removal of gloves. No eating, drinking, applying cosmetics, or handling of contact lenses is permitted in the work area. ...read more.

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