• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

To determine if temperature would, affect the respiration rate of immobilised and non-immobilised yeast cells.

Extracts from this document...


To determine if temperature would, affect the respiration rate of immobilised and non-immobilised yeast cells. Martha Orotayo Prediction: That the optimum rate of immobilised yeast cells would be greater than that of non-immobilised yeast cells due to changes in the temperature. Hypothesis: As I am investigating the effect of temperature upon the respiration rate of immobilised yeast cells its essential to know any relevant background information on yeast cells. Immobilisation is termed as the act or process whereby you prevent a thing from moving. In terms of speaking of immobilised yeast c ells it would men that these cells would be unable to move about. Also the movement in space of an immobilised enzyme is restricted either completely or is limited to a small region. Yeast is composed of proteins and celluloses (much more celluloses involved) and also has a tough outer shell made up of protein and cellulose. Another name for yeast cells is Saccharomyces cerevisiae (meaning a mould that ferments in cereal the sugar to make alcohol and carbon dioxide) which has come to be an important model into the study of Eukaryotic cell's mode of life. ...read more.


7. Then place this beaker underneath the syringe. 8. Allow beads to stand for 20minutes in the calcium chloride solution. 9. Set up water bath to temperature of 20�C. 10. Once beads are well formed then strain with a tea strainer to separate the beads from the Calcium Chloride solution. 11. Measure 200cm� of 3% sucrose solution into a 250cm� conical flask. 12. Set up the water trough with correct apparatus. 13. Place the conical flask into the water bath. 14. Measure the amount of gas produced. 15. Repeat steps 11-14 for different temperatures. Result table Temperature Immobilised yeast cell (cm�) Non-immobilised yeast cell (cm�) 20 �C 1.1 2.1 25 �C 1.5 1.6 30 �C 3.0 4.3 35 �C 4.0 4.1 40 �C 8.6 1.1 45 �C 10.5 6.6 50 �C 11.6 14.6 Conclusion In conclusion, it appears that the immobilised yeast cells were most effective for this particular experiment because they appear to respire the most within the given time. Also it would appear that the optimum temperature for the immobilised yeast cell was 50�C as the gas collected at it was highest at 11.6cm�. While non-immobilised yeast cell had an optimum temperature of also 50�C but the amount of gas collected was higher with 14.6cm�. ...read more.


I had to use an electrical apparatus (i.e. the water bath) and this carries the potential hazard of giving an electric shock which may be fatal following contact with live components. I had to put into consideration not to tamper with electrical equipment to attempt repairs and to previously check that it was unsafe in any manner. I also checked the re-test dates on it, kept water away from mains electrical sockets. The shards of the broken conical flask were disposed of immediately as they could have caused severe physical injuries. Broken glassware is never cleaned up by hand. Instead dustpans and brushes were used to collect the broken glass and it is disposed of appropriately. I also made sure not to use any damaged glassware. The sucrose solution is considered to be toxic to the central nervous system can induce confusion, fatigue, irritability, and other behavioural changes. I organised my work around my work top to reduce exposure to hazards. Non-latex gloves would be worn during the experiment were I to have any broken skin or rashes on my hands were present during the experiment. Hands were washed after viable materials are handled and before leaving the laboratory and at the conclusion of the experiment. Hands are washed following removal of gloves. No eating, drinking, applying cosmetics, or handling of contact lenses is permitted in the work area. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Energy, Respiration & the Environment section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Energy, Respiration & the Environment essays

  1. Marked by a teacher

    An investigation into the effect of different sugars on respiration in yeast.

    5 star(s)

    When the sugar concentration is increased, the rate of reaction will increase up to a point, when the rate becomes constant even if more substrate is added. This is because the enzymes in the yeast are saturated with substrate. I have found that out of the six sugars I am

  2. Marked by a teacher

    Effect of Anaerobic Respiration On Yeast

    5 star(s)

    On the other hand if the temperature is too high then this can cause denaturing of the enzymes. This means that the shape of the active site can change within some, or possibly all, of the enzymes. This therefore means that the substrate molecules can no longer fit into the

  1. Marked by a teacher

    effect of temperature on the rate of respiration in yeast

    5 star(s)

    If the disruption occurs at or near the active site, the active site becomes distorted and substrate can not fit perfectly. Thus not all enzymes in the solution will be able to catalyze their reaction. With increasing or decreasing pH, more enzymes become denatured, and fewer enzymes are able to form that enzyme-substrate complex.

  2. What effect does substrate have on respiration in yeast?

    Graduated cylinder needs to be fully filled to make sure the readings obtained are the actual amount of carbon dioxide produced. 5. Use a clamp stand to hold the inverted graduated cylinder in place. 6. Attach one end of the rubber tubing to the single hole in the rubber stopper.

  1. An investigation into the effects of temperature on the rate of anaerobic respiration of ...

    0 5 1 0 7 4 3 1 2 2 0 2 10 2 0 9 7 5 2 3 3 1 5 15 2 1 10 8 6 3 3 4 3 7 20 2 1 10 8 7 4 4 4 4 12 25 3 2 10 9

  2. Investigate the effect of bile salt concentration on the digestion of milk by the ...

    While data may be precise in that is it consistent it is not necessarily the correct value due to errors which alter it. Additionally, data may be accurate but over a wide range of value and therefore statistical methods are needed to determine the true experimental value such as the mean and so this is also not valid as well.

  1. the effect of bile concentration on the activity of the enzyme lipase during the ...

    temperature is a variable we have to control pH Probe Used to measure the pH change whilst the reaction is taking place. Accurate and way of measuring pH because pH probe will be set to pH 7 by using a pH buffer of 7 so the probe will be accurate

  2. Investigate the effect of changing the sugar concentration on the rate of respiration of ...

    I will test varied concentrations of sugar solutions throughout my experiment. The outcome variable will be the rate of which respiration takes place. By changing my input variable (concentration of sugar solution) the output variable (rate of respiration) will be altered.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work