To determine which solvent system gives the best resolution of separation of the individual amino acids.

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Key:

Ds represents the distance moved by the solvent up the chromatography paper

Da represents the distance moved by the individual amino acid up the chromatography paper

Rf represents the Rf value of the individual amino acids, that is, (Rf = Da / Ds)

The asterix, * indicates the uncertainty in the values calculated, which is, + 0.05cm.


Key:

Ds represents the distance moved by the solvent up the chromatography paper

Da represents the distance moved by the individual amino acid up the chromatography paper

Rf represents the Rf value of the individual amino acids, that is, (Rf = Da / Ds)

The asterix, * indicates the uncertainty in the values calculated, which is, + 0.05cm.

Bases of the evaluation:

To determine which solvent system gives the best resolution of separation of the individual amino acids, the following points would be taken into consideration:

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  1. The spots of the individual amino acids either alone or in the mixture, must be visible. The solvent system, which gives the best clarity of the individual amino acids, would be the best.
  2. The distinct nature of the Rf values calculated for the individual amino acids alone. When I say distinct, this means that the spots should be at varying distances from the origin line, with the values for their distances being very different. Their values should not be too close and as such give a bad resolution.
  3. An equally distinct nature of the Rf values calculated ...

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