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To find the optimum temperature that the enzyme catalyse works at.

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Biology SC1 investigation Intro Aim: To find the optimum temperature that the enzyme catalyse works at. Introduction: the livers' main purpose is to break down things that the body needs. The cells in the liver help the blood to join with food substances and excrete excess waste materials and toxins, as well as such substances as estrogens, steroids and various other hormones. The cells used to break down these substances are known as enzymes. The main used enzyme is called catalase. The enzyme catalase can only work at a specific temperature and needs several other things to achieve maximum efficiency and potential. Using a piece of sheep's liver, which has similar qualities to human liver, as well as containing the enzyme catalase, we can find the temperature that optimises catalase. Apparatus: Water Bath Equipment: Bunsen Burner Heat Proof Mat Gauze Tripod Beaker Test Tube Thermometer Other Equipment: 5 pieces of liver Hydrogen Peroxide Test tube rack Test tubes Stopwatch Tweezers Weighing Device Cutting equipment Diagram Method / prediction Plan: to find the optimum temperature for the enzyme catalase to work at the pieces of liver containing the enzyme will have to be heated to a variety of different temperatures. I will then have to have a reaction that can be controlled to measure the efficiency and speed of the reaction. Catalase reacts violently with hydrogen peroxide, therefore to get a reaction I shall put the liver containing catalase into some hydrogen peroxide. ...read more.


The timing shall commence as the liver touches the hydrogen peroxide and stop the timing as the frothy liquid gains enough mass to reach the top of the test tube or stops getting any higher up the sides of the test tube. Once I have got all the results from the range of temperatures I have tested, I will do some of the temperatures again to show repetition has occurred and that the results were not a fluke or anomaly. Then with these newly acquired results, I shall build a table of results and some graphs to display what temperature the enzyme worked best at. I will not be able to get a precise reading but the answer should be inside a range of 5 degrees Celsius. Safety: there are many safety aspects in the procedure of this experiment that I must consider before starting. When cutting the liver I should be very careful with the blades that will be used, if my skin makes any contact with the liver or I bled then the skin should be washed immediately. When using the water bath there are several things to be careful of. The Bunsen burner will be on a very hot setting so all clothing should be out of the way or tucked in so nothing will set alight. The hot water in the beaker should be treated with caution, as it will seriously burn the skin if it is spilt onto the skin. ...read more.


The multiple results seem to correlate and show up no anomalies. This graph shows the averages of the tests that had multiple tests done. It shows a very clear trend. It shows that the enzyme works, but not very well at the 0 - 20 degrees mark. At around 30 - 40 the results drop and the enzyme is at its most efficient. As the temperature rises the enzymes reaction time decreases more and more. By the time it gets to boiling point the enzyme is working very slowly indeed. This could be because it has been denatured or at least partly denatured. Evaluation Accuracy: The experiment went very well and all of the results gathered seemed to be accurate according to the trend that developed in the graphs. I was not able to do multiple tests at the 45-50 mark, the results I do have do fit but the average is not as accurate as I would have liked. Improvement: the number one improvement I would make if I did the experiment again is to get more results to go towards a more accurate average graph. The readings could have been more accurate if better equipment was open to us like automated liver droppers and laser sensor timers. Overall though I am happy with my experiment as it managed to show a clear trend and the temperature that the enzyme worked best at. Alex Walker Biology Sc1 Investigation 1 11Rw ...read more.

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