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To Investigate an Enzymes Reaction.

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TO INVESTIGATE AN ENZYMES REACTION Introduction In this investigation I am trying to find out how a substrate concentration can affect the rate of reaction of the catalase, (catalase is the fastest enzyme known). Enzymes make reactions happen at a much faster rate and come in two main types; Breakers (break up large molecules into smaller ones) and Builders (joins small molecules together to make large ones). Enzymes are specific, they will only act on one particular substrate as the substrate has to fit the enzyme's active site. This diagram shows how a breaker enzyme works: Here is how a builder enzyme works: For my experiment, the substrate I am going to use will be Hydrogen Peroxide (H2O2) and I will extract the enzyme from soaked peas that have been peeled, split and crushed using a pestle and mortar. The peas contain an enzyme called catalase and this is what causes the breakdown of hydrogen peroxide into water and oxygen speed up. Input and Outcome I will be changing the concentration of Hydrogen Peroxide (the substrate) by mixing it with water each time. I will use six different concentrations of the substrate and go down in the amount of percentage used. ...read more.


5. Repeat the whole of the experiment twice more. I am repeating the experiment so I can see if I have any slight changes in repeating the experiment, also to ensure that there wasn't something wrong with the first amount of peas used, or substrate. Results Table Result A Height (cm) after:- Test tube Concentration Of Substrate Percentage Of Water 20 secs 40 secs 60 secs 80 secs 100 secs 120 secs 1 100% 0% 2.3 2.8 3.0 3.3 3.6 4.0 2 80% 20% 2.4 2.7 2.9 3.2 3.5 4.2 3 60% 40% 2.2 2.5 2.6 2.9 3.4 3.7 4 50% 50% 2.5 2.7 2.8 3.0 3.2 3.5 5 40% 60% 2.1 2.2 2.4 2.7 2.8 3.0 6 20% 80% 2.0 2.3 2.3 2.5 2.6 2.6 I have not done a test for 0% of H2O2 as I know that the reaction of the peas with the water would not take place. This is because water does not react with the crushed peas in the way that I want it to for my experiment and also the experiment is partially about the hydrogen peroxide and catalase reaction. ...read more.


I think that my method was a good one although I think that I could have added one more thing and that would be to measure the temperature of the area I was working in and the temperature of the substrate I was adding to the enzyme as enzymes are affected by temperature as well. When enzymes reach a certain hot temperature the proteins break down and the enzymes active site changes and the enzyme has been de-natured. But as the temperature rises to the certain height, the reaction does speed up so next time I could also monitor the temperatures. I don't feel that my results were accurate enough, even though I repeated the experiment three times, to give an accurate conclusion or an accurate graph. I feel that they were affected by some other form of enzyme effects. If I am to repeat the experiment I need to look at the temperature and the pH as I think that it will come down to these being different. This would be the reason why the results are not accurate and why they seem to be different each time I have repeated them. ?? ?? ?? ?? Enzyme Experiment Biology Coursework Vikki Stabler 11M4 - 1 - 02/05/2007 ...read more.

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