To investigate the effect of changing concentration of Hydrogen Peroxide (H2O2) on the enzyme catalase.

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Ross Murray

        To investigate the effect of changing concentration of Hydrogen Peroxide (H2O2) on the enzyme catalase.

Equation: -  

2H2O2          catalase           2H2O + O2

Background information: -

        My background information on my experiment is that the substrate (H2O2) is by product of the chemical RX inside the body that is linked to the metabolism; the substrate is a toxic (poisonous) by product of the RX chemical, which kills and stops RX’s. The H2O2 that we are going to use has been broken down into safer less toxic molecules and compounds.

        Enzymes are chemicals/catalysts. Catalysts alter the rate of reaction by making the rate of reaction faster. Catalase is a protein, which means this is an enzyme because all enzymes are proteins. Enzymes being proteins the work best at 37 degrees (body temperature) to much excess heat and it will denature the enzyme, the more enzymes you have the faster the rate of reaction will be.

Lock and Key Theory

        +

Catalase                Substrate                                        

(Enzyme)                (H2O2)                        Complex        Enzyme        Product

                

Rate        Max rate                      Rate

                        

         Substrate concentration                                      Enzyme concentration

                Substrate concentration                     Enzyme concentration

                reaches a Plateau                        plenty of Enzyme

                because all Enzyme                        Molecules to deal

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                Molecules used up                        with substrate.

                so you add more                          Substrate is constant.

                H202 the rate stays                        

                the same.

                Plan - How are you going to do the experiment?

        In my experiment I am going to use the catalase yeast because it gives a faster rate reaction, also I will be using a solution of H2O2, which comes neat 6% and 40 volume.

A table of results to show the percentage of water

                                

The temperature that will be used will be room temperature and

The size of volume that will be used will be in 30cm3 portions

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