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To investigate the effect of temperature on the enzyme catalase.

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Introduction

To investigate the effect of temperature on the enzyme catalase Aim: To investigate the effect of temperature on the enzyme catalase Apparatus Celery extract (catalase) Labels 20 Volume hydrogen peroxide Distilled water 6 100cm beakers 20cm and 50 cm measuring cylinders Balance Stopwatch Trough Clamps & stand 150cm conical flask + bung + tubing + 3 way tap 10cm syringe Beehive shelf Gas syringe Goggles Water bath at 25 & 35�C 250cm beakers & thermometer for water bath Method 1.Weigh out 1 sample of celery extract of 10g in a conical flask. 2.Place in a water bath at 25�C. 3.Place about 20cm hydrogen peroxide solution in a container in the same water bath. 4.Leave for 10 min to equilibrate. 5.Set up the apparatus as follows: -Fill a trough with water -Fill a measuring cylinder with water and invert in the trough (ensure that no air bubbles are in the water) -Secure the cylinder with a clamp & stand -Position the bung and 3 ways tap to fit on a conical flask, ensure that this can be held in place with a clamp & stand -Put the end of the tubing in the measuring cylinder 6.Make sure that the tap is closed and fill a syringe with 10cm hydrogen peroxide from the water bath. ...read more.

Middle

Time/Temperature 15�C 25�C 35�C 45�C 55�C 30second 14.00ml 16.00ml 17.50ml 19.00ml 8.00ml 60second 22.00ml 23.00ml 25.00ml 32.00ml 15.00ml 90second 29.00ml 31.00ml 39.00ml 45.00ml 17.00ml 120second 34.00ml 35.00ml 50.00ml 54.00ml 21.00ml Table 2. Table 3 (below) shows the results obtained when the experiment was conducted for the tired time. Time/Temperature 15�C 25�C 35�C 45�C 55�C 30second 10.00ml 11.50ml 14.00ml 13.00ml 9.00ml 60second 13.00ml 16.00ml 21.00ml 26.00ml 12.00ml 90second 15.00ml 21.50ml 27.50ml 33.00ml 14.00ml 120second 18.00ml 26.00ml 33.00ml 44.00ml 16.00ml Table 3. Average: Table 4 shows the average taken from the results of the above three experiment Time/Temperature 15�C 25�C 35�C 45�C 55�C 30second 9.33ml 11.83ml 14.17ml 16.67ml 7.33ml 60second 14.17ml 18.33ml 22.67ml 28.17ml 11.33ml 90second 18.67ml 24.83ml 31.33ml 37.67ml 13.83ml 120second 22.17ml 30.33ml 39.33ml 47.50ml 16.83ml Table 4. The following calculations shows the volume oxygen gas produced per second for each temperature for the first 30 seconds of the experiment. The first 30 seconds were chosen for this because the volume of oxygen produced during this time can be measured most accurately. 15�C: 9.33ml / 30sec = 0.31ml/sec-1 25�C: 11.83ml / 30sec =0.39ml/sec-1 35�C: 14.17ml / 30sec = 0.47ml/sec-1 45�C: 16.67ml / 30sec = 0.57ml/sec-1 55�C: 7.33ml / 30sec = 0.24ml/sec-1 Conclusion The enzyme molecules have active sites that help them to recognize their substrates in a very specific way. ...read more.

Conclusion

Evaluation The most important steps were adding enzyme solution into the conical flask and measuring the volume of oxygen gas produced. They are very easy to cause errors, on one accession, the apparatus was not checked before the experiment started, and as a result a leak occurred. So make sure that the apparatus is securely assembled, because the pressure of gas generated may cause the joints may leak if otherwise. After adding the catalase to the hydrogen peroxide solution (in the conical flask), the 3-way tap should be turned off immediately, so that no oxygen can escaped form the conical flask to the syringe. (The one we use to add catalase) Another important factor could cause error is the temperature of the solutions, because the thermometers gives +/- 0.5�C of error. The following measuring was identified as being important for ensuring accuracy whilst carrying out the experiment as well. 1.When hydrogen peroxide is measured in the measuring cylinder, care must be taken to make sure that the reading is taken from the base of the meniscus, because only by this means will read be accurate. 2.Care should be taken to ensure that the plunger of the syringe that is used to deliver the catalase is fully depressed so that the full volume of enzyme is added to the hydrogen peroxide. ...read more.

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This is a reasonably good account of the investigation but greater attention to detail is needed at A level to gain high marks. The author does not discuss variables carefully and more A level standard theory could have been used to help explain the results.

Marked by teacher Adam Roberts 05/09/2013

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