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To investigate the effects oftemperature on catalyse action?

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To investigate the effects of temperature on catalyse action? P PLANNING The Problem The problem which I intend to investigate is the amount of oxygen formed, and whether the temperature affects this. Scientific Background Enzymes are biological catalyse. They speed up chemical reactions in all living things, and allow them to occur more easily. The fastest enzyme known is called catalyse. It breaks the chemical hydrogen peroxide down to water and oxygen. Catalyse is found in all cells and protects them from the dangerous waste chemical. Each particular enzyme has a unique 3-dimensional shape shared by all its molecules. Within the shape there is an area called the active site where chemical reaction occurs. The substrate (hydrogen peroxide) and the catalyse molecules are continuously on the move. Every so often they will collide so that the substrate molecule(s) fits into the enzyme's active site. Then the substrate is broken down into the two products The enzyme is not used up in the reaction. It is ready to work again. No matter what job an enzyme does it will work in a similar way. The formula for this experiment is: Hydrogen peroxide --> oxygen + water 2H2 O2 --> O2 + 2H2O This information is found from www.s-cool.co.uk Experiment This is how my apparatus will look like: Apparatus The apparatus which I will be using for this experiment are: -Side arm conical flask -Stop clock- time for 60 seconds -Bungs -Measuring cylinder. -Hydrogen Peroxide- 15ml -Tile -Kettle. ...read more.


My overall results should be averaged so that it should be more accurate. Prediction I predict that the higher the temperature, the faster the time of catalyse to react. However, when the temperature reaches it optimum level it will start to slow down. This is how I think my graph will look like: At low temperature, the enzyme does not have enough energy to meet many substrate molecules, so the reaction is slowed. At the optimum temperature, the enzyme is most efficient. This is between 40�c -60�c At higher temperature, the structure of the enzyme is broken down. The enzyme cannot be used again The graph shows that when the rate of the enzyme activity reaches its optimum level its starts to decrease, this is because the enzyme denatures. This information was found from www.s-cool.co.uk Explanation This is because; increasing the temperature makes the particles move faster. This leads to more collisions. Also the particles have more kinetic energy, so more collisions will lead to reaction. Catalyse works only under particular conditions. It works best at 37�c. At higher temperatures the protein structure is permanently changed (denatures): it no longer decomposes hydrogen peroxide. O OBTAINING RESULTS Table of Results First results Temperature �c volume of 0� formed, after one minute, ml 0 8.0 20 11.0 40 12.0 60 17.0 70 7.5 Second results Temperature �c volume of 0� formed, after one minute, ml 0 8.0 20 10.0 40 11.0 60 18.0 70 8.0 Average of all results ...read more.


Factors which may have caused this could be that, like the wind form the open windows, the temperature of the boiled water may not be the same readings at the start of the experiment, also the iced water may not be exactly at 0�c, ad the readings collected may not have been at the correct time which is exactly 60 seconds. I repeated my results, and averaged this out to get an accurate and reliable result as possible. I approximated my results to one decimal place, therefore I think that my results are reliable. The range used was a wide range which made it even more reliable. Further Work Changes to my experiment which may have helped me get better results will be to go up in 10�c, this is because the range will be closer making the results more accurate and reliable. Any other alternative experiment I would do to help me investigate the problem is to compare with potatoes and liver, rather than just potatoes, this is because both of these things contain catalyst for this reaction, and comparing both substances would make my experiment more reliable. Extending the range of my experiment would not help me obtain more reliable results because I think that the range is a good range to have as it is not too small or too big. Although using 10�c would be time consuming, but the results will be more accurate. ?? ?? ?? ?? Chinyere Akosim ...read more.

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