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Trypsin Enzyme Investigation

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I'm going to investigate the enzyme which is trypsin. The protein I'm using is glue which sticks the black silver salts to the x-ray film. If you get a splinter and cut a little split at one of the ends and slide the x-ray film in. Get a beaker and fill it half away with cold water also get a test tube and pour a little dash of trypsin in. Place the test tube into the beaker and put the splinter into the test tube. Every so often take the splinter out of the test tube and wait until you get black on your fingers. If you get black on your fingers that shows trypsin works. The x-ray film should go clear because the glue was digested by the trypsin. Enzyme is one of the many specialized organic substances, composed of polymers of amino acids, that act as catalysts to regulate the speed of many chemical reactions involved in the metabolism of living organisms. ...read more.


I chose these temperatures approximately 70'C, 50'C, 20'C and 10'C. I put the kettle on because I needed boiling water to make the high temperature which is 70'C. I got 5 ice cubes and put them in a beaker, I then poured 250ml of cold water in the beaker. The water was boiled so I got another beaker and poured 250ml of boiling water. In another beaker I poured half of cold and boiling water which will approximately make 50'C. In the last beaker I just poured 250ml of warm water. I then got 4 test tubes and poured 5ml of trypsin in each one and then I placed them into the beakers. I got 4 splinters and cut little splits at one of the ends and slide the photographic film in. My friend Samantha and I were working together to do this experiment. Samantha got a stop and press start when I place the splinters with the photographic film in the test tubes. ...read more.


Cleared B 38'C Yes Cleared C 29'C Started to Change D 6'C No Change A 43'C Cleared B 30'C Cleared C 28'C Almost Cleared D 5'C No Change Result table 3 Test Tube Temp of water Temp every 5 minutes Condition of film A 65'C 59'C Yes Started B 41'C 39'C Yes Started C 33'C 32'C No Change D 9'C 8'C No Change A 53'C Almost Cleared B 38'C Almost Cleared C 32'C Yes Started D 7'C No Change A 49'C Yes Cleared B 36'C Yes Cleared C 32'C Almost Cleared D 6'C No Change A 44'C Yes Cleared B 32'C Yes Cleared C 29'C Yes Cleared D 5'C No Change Result table 4 Temp'C Time taken for film to Clear (min) Average Time (min) 65 1. 20 17 65 2. 15 17 65 3. 15 17 41 1. 20 17 41 2. 15 17 41 3. 15 17 33 1. 20 17 33 2. 15 17 33 3. 15 17 9 1. 20 20 9 2. 20 20 9 3. 20 20 ?? ?? ?? ?? ...read more.

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