Various Factors in the Preparation and cooking of Fruit and Vegetables can change the Vitamin C content. Devise and experiment one of those factors.

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Various Factors in the Preparation and cooking of Fruit and Vegetables can change the Vitamin C content. Devise and experiment one of those factors.

Pilot Method

  1. Use a syringe (or Measuring Cylinder for measuring equal amounts) and pour out 10ml of DCPIP (Solution used to detect Vit.C) into a small beaker.
  2. Using a separate Syringe, draw up 10ml of solution 0.1% Vitamin C
  3. Slowly add the Vit.C into the DCPIP solution beaker, swirling around a few times. (swirling helps solutions to mix)
  4. Continue to add the Vit.C until DCPIP turns a permanent straw colour.
  5. Note the amount of Vit.C it took to change the colour of the DCPIP.
  6. Repeat steps 1-5 5 3 times and take an average.

Pilot Results

Strength of DCPIP X Volume = Volume of Vit.C x Strength

)(  x  10ml = 8.5ml  x 0.1%

)( = 6ml x 0.1%

     10ml

Strength of DCPIP = 0.06

Strength of DCPIP X Volume = Volume of Vit.C x Strength

)(  x  10ml = 6ml x 0.1%

)( = 6ml x 0.1%

    10ml

Strength of DCPIP = 0.06

Strength of DCPIP X Volume = Volume of Vit.C x Strength

)( x 10ml = 6ml x 0.1%

)( = 6ml x 0.1%

      10ml

Strength of DCPIP = 0.06

Average Strength of DCPIP = 0.06

Method

  1. Using a Syringe (or measuring cylinder) put 10ml of DCPIP into a small beaker.
  2. Using a separate syringe, take up 10ml of Juice (Juices will change per experiment)
  3. Slowly add the juice a little at a time until the DCPIP turns a permanent straw colour.
  4. Make sure 1oml is used at all times as to keep it a fair test at all times.
  5. Repeat experiment 5 times on each of the 4 different Juices chosen and take an average one completed.
  6. Put all recordings and measurements in a chart, and using the Equation below finding the strength of the Vit. C in each Juice type (Strength of DCPIP taken from Pilot experiment)
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Strength of DCPIP X Volume = Volume of Vit. C x Strength

Results

Juice 1  =  Ascorbic Acid. (Pure Vitamin C)

Juice 2  =  Orange Juice. (Smooth)

Juice 3  =  Pure Orange with Mango Pure.

Juice 4  =  Orange and Grapefruit.

Ascorbic Acid (J1)

Strength of DCPIP x volume = Strength of Vit. C x Volume

0.06           x   10ml   =      )(                x    8ml

)(  =  0.06 X 10ml

          8ml

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