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Vitamin C content in juice

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Introduction

Is High Vitamin C all it claims to be? These days, there is much concern on our consumption of food drink, we're told we do not intake the required daily level of fruit and veg, many manufactures claim to provide excellent levels of nutrients, This experiment will be carried out to see whether the vitamin c levels claimed by manufacturers are actually present in the drinks, this will be done by experimenting various drinks using blue DCPIP solution. The quantity of vitamin C in food and drink can be determined using a simple colour test. Vitamin C decolourises the blue dye DCPIP (dichlorophenolindolphenol). Vitamin C is an antioxidant and reduces the DCPIP. DCPIP changes from blue to colourless (or slightly pink) as it becomes reduced. Hypothesis: Is High vitamin C all it claims to be? Prediction: Fruits have high levels of vitamin C therefore fruit juice, in particular orange will require the least volume to decolourise DCPIP as Orange juice has the most Vitamin C. Ethical issues- The nature of this experiment is unlikely to have any ethical issues, the aim of the results is increase understanding of our diet and nutrition levels. ...read more.

Middle

Therefore, in this experiment, we will be looking for the volume of drink required to decolourise the DCPIP. Risk assessment- apparatus Why it will be used/suitability Possible risks Precautions/changes Fruit juices Used measure Vit C content, consumed by people. Date expiry causing bacteria. Kept in cool condition to ensure there is no affect on the variable, DCPIP 1% Used to test the juice in its Vit C content. Spillage, staining, Used carefully in measurements. Water Pipette Used to measure the volume of juice. Incorrect measure can affect validity of results. The intervals of the pipette can be decreased to make more precise and accurate readings. Test tube To put the decolourising liquids in. Smash spill, destroy experiment. Handled with care and precision. To make the results of the experiment more accurate and reliable, the volumes of juice and DCPIP will be measured carefully with precision; the apparatus will also be changed to a higher degree of accuracy. All safety procedures will be done, the workplace will be kept clean and safe, the test tubes will be handled with carefully and DCPIP will be kept within the edge so it does not spill. Results Volume of juice required to decolourise of DCPIP (cm�) ...read more.

Conclusion

This could be because of limitations in our apparatus and procedure. The interval size on our apparatus such as the pipette was not very accurate; furthermore there was no accurate digital method of looking at the decolourisation by DCPIP. Moreover random errors or leniency in measuring and generally the time restriction may have affected our results. These were limitations in our experiment, which could be reversed to produce more accuracy in our results. Summary Overall my results were reliable and valid but they could be improved to produce very precise and accurate results. The equipment used in this experiment could have limited my results. More accurate equipment could have been used to produce more accurate results such as using burettes or graduate pipettes rather than dropping pipettes. Automatic burettes measure to one decimal place whereas dropping pipettes measure to 1 ml and so burettes are more accurate when measuring volumes. Also, our time could have been extended to another lesson or an hour or so, so it could have been done in a relaxed and therefore precise and careful manner, which would have improved our results in terms of accuracy and precision. We would then have more options and hypothesis to look at with our set of results. ?? ?? ?? ?? Faisal Maljee Ms McDonnell ...read more.

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