1)        What does the word ‘diuresis’ refer to?

Diuresis refers to the increase in urine flow that are caused by diuretics which in turn causes higher urine release.

2)        What are the particular characteristics of a ‘water diuresis’?

Water dieresis is the excretion of urine after drinking water; results from reduced secretion of the antidiuretic hormone of the neurohypophysis in response to the lowered osmotic pressure of the blood.When there is an increase in water uptake, hence the plasma osmolarity increases. The osmoreceptors in the anterior hypothalamus would then be suppressed and causes less ADH to be secreted from the posterior pituitary gland. This decreases the water permeability of the distal tubule and collecting duct causing lower water absorption. Besides that, the urine osmolarity would also decrease to a value lower than 30mOsm/kg H2O and there will be an increase in urine which is diluted.  

3)        In the table below record the details for each of the subjects in your group.  If there were only 3 subjects in your group draw a line through the last row (Subject D).

Calculation:

Volume of water ingested = body weight × 12mL

= 70.5×12mL

= 846ml=0.846L

4)        Complete the data tables on the next two pages.  There should be one table completed for each subject in your group.  One subject will have data for urine osmolality but not ion concentrations.  The other subjects will have data for urine ion concentrations but not osmolality.  If there were only 3 subjects in your group write ‘no subject available’ across the last data table.

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5)        For the subjects in your group plot graphs showing the effects of the various treatments on:

  1. Rate of urine production (ml/min) versus time.  Plot the data for all subjects on the one graph.  Make sure you identify each subject

  1. Urine osmolality versus time.  There will be only one subject for this graph.

  1. Urine Pottasium concentration versus time.  There will be 2 (or 3) subjects for this.

  1. Pottasium excretion rate versus time.  This will be for the same 2 (or 3) subjects as above.

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