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What Are the Advantages and Disadvantages of A Light And Electron Microscope?

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Introduction

WHAT ARE THE ADVANTAGES AND DISADVANTAGES OF A LIGHT AND ELECTRON MICROSCOPE? The naked eyes are not powerful enough to see any type of ultra structure, such as a living or dead cell and its components. So, to further scientific stuffy the microscope invented; the light microscope. The light microscope, relatively small and light weight to the electron microscope, is the earlier discover of both types of microscopes, which I am going to discuss today. As with everything it has its advantages as well as its disadvantages. This certain microscope can magnify any sample up to 2000 times its original size. This is useful for scientists because if the species were alive you can monitor movement of the cell. The magnification allows the cell structures to be observed too. The magnification means that it gets bigger, so when you focus the knob it becomes larger. Furthermore, the light microscope produce an image which are I colour. When I stained a specimen on my slide for microscopy, I saw the colours which were very vivid. ...read more.

Middle

The background of light is that, light can not pass through anything half its wavelength. For instance, white light is 0.55µm and anything smaller than 0.275µm is way too small for light to sufficiently pas through. Therefore this means to achieve resolution for a finer and more detailed image something else should pass through the gaps; something smaller than photons. This is where the electron microscope comes to play. This recent finding uses electrons, as they are miniscule, to give a higher resolution and with that comes greater detail. The electron microscope is relatively larger and uses a vacuum and electrons to produce a high quality image, by sending electrons through the tiny gaps. (An anode is there to speed it up). This gives an advantage to the electron microscope, a better resolution. As a result if any person wants to see something such as the mitochondrion, the Golgi Body or even a lysosome they are able to. ...read more.

Conclusion

Clearly if you had air, the electrons will knocked by the air particles and it has to be in that magnetic field. This is the reason why it has to be dead. To conclude, light and electron microscope are both equally as useful as well as being limited in their own way, it recommend that in order to get the more satisfying ultra structure image to use the electron microscope and the light one for something less accurate and more general. To finish I am going to draw up a table, of advantages and disadvantages of each microscope- for an easy reading. LIGHT MICROSCOPE ADVANTAGE DISADVANTAGE Small No more detail once reached a certain point Light weight Magnification up to 2000x Sample can alive or dead Colour ELECTRON MICROSCOPE ADVANTAGE DISADVANTAGE Great resolution and magnification for details like the ultra structure Heavy 200,000x magnification Big You can see a whole exterior of a specimen- its body Mono colours. Black and white You can see the inside Requires skill, expertise and training or experience. Sample has to be dead Very complicated. Movemtn can not be registered By Aswani Pillai 6L3 ...read more.

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Response to the question

The comparison of advantages and disadvantages is done very well but the level of detail is lacking. The student simply tends to state the advantages and disadvantages and for A level, I believe they should have explored deeper into the ...

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Response to the question

The comparison of advantages and disadvantages is done very well but the level of detail is lacking. The student simply tends to state the advantages and disadvantages and for A level, I believe they should have explored deeper into the reasons behind it. Why is the microscope limited in the way that it is? How does it work on a fundamental level? I think such questions are key to answering the question fully and appropriately for this level. Their response is very clearly written but not necessarily clearly presented. I feel light blue is not the best colour to use for a piece of work like this.

Level of analysis

The student's analysis is poor for A level. They do not explore deeply enough into the reasons behind how the microscopes work by explaining it using fundamental physics that they have learned from the syllabus. Their judgements, however, were suitable and the relevant evidence was used to support it even if it was not supported by detailed evidence. To improve, they need to go back and explain why - re-reading over their work they will find that there were many areas where the question of "why?" or "and?" arose. Such questions should not occur if a piece of work answers the question fully.

Quality of writing

There was a typo in the work. This shows the lack of proof reading at the basic level as the word processor should pick this up automatically. The appropriate technical terms were used but the student needs to show a proper understanding of such terms by either defining them in a glossary or explaining them in the body of the work. The student follows the typical layout of the work, although they do not follow the typical presentation of the work. The colours are not best suited to a piece of coursework like this.


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