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# What Effect Does The Temperature Of Water Used Have On The Rate Of Coagulation Of Milk And Rennin Solution?

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Introduction

What Effect Does The Temperature Of Water Used Have On The Rate Of Coagulation Of Milk And Rennin Solution? In this investigation I am going to mix a certain amount of rennin solution and milk, and record how long it takes the mixture to coagulate. I will repeat this procedure of mixing the rennin and milk when they are heated to 5 different temperatures and will leave them to coagulate in a water bath so as to maintain their temperature. I will repeat each temperature 3 times, calculate an average time of coagulation for each temperature and I will hopefully find a correlation between the temperature of the mixture and the time taken to coagulate and therefore come to a conclusion to my title question. I will use my existing scientific knowledge to make a prediction and to help me understand and explain the outcome once I have gained my results. Enzymes are biological catalysts. They speed up a reaction without affecting the nature of the end products. Enzymes are specific and an enzyme will only work with a particular substrate. Rennin is an enzyme. So in the context of this experiment the enzyme, rennin, is speeding up the reaction of coagulation when the enzyme solution is mixed with milk. ...read more.

Middle

I expect this optimum point to be about 40 degrees Celsius, as this is most animal/human enzymes optimum temperature. This will be shown in my experiment if the mixture that is quickest to coagulate is that nearest to 40 degrees Celsius. Safety It is imperative that the experiment I carry out is done so in safe conditions. There are measures I can take to ensure that this is the case. * All coats and bags must be well out of the way of the area being used, as they may cause someone to trip over them causing potential injury. * All liquid must be handled carefully so as to prevent spillages as this could cause potentially dangerous slippery surfaces. If something is to be spilt, it is to be wiped up immediately. * Glass beakers/test tubes will be in use so careful handling is vital. If something is to smash, it needs to be cleaned up immediately, yet cautiously. * There should be no running in the lab at any time. * As electronic water baths are being used extreme care must be taken, as spillages of water near electrical sockets can be highly hazardous. * Although the enzyme solution is a low dilution, it may cause an allergic reaction so avoid skin contact and rubbing eyes with contaminated hands. ...read more.

Conclusion

This includes the same water baths, syringe, pippet, thermometers, stop clock and black card. This ensures that if an error occurs due to a discrepancy of a piece of equipment, the same error will occur on all tests. * The temperature of the water baths must be that of what is stated in the method. Measured amounts must also be precise, as discrepency in measurements could lead to anomolous data. * All timings must stay consistent and exact throughout the investigation to make sure all the results are to the nearest 30 seconds. To ensure this is a fair test there must only be one variable factor. In this investigation the variable factor will be the different temperatures used. I will keep all the other factors the same throughout. The concentration of rennuin solution will remain constant throughout (at 1%) as I will take all the measured samples of rennin solution from the same beaker of solution. When I have completed my experiment, I will use my obtained results to calculate an average time of coagulation for each temperature. I will then use these averages to plot a graph to show the effect of temperature on the activity of the enzyme rennin. I will then be able to assess the accuracy of my prediction and the success of my experiment as well as come to a conclusion of the initial question. ...read more.

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