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What is the effect of changing the concentration of Sodium Chloride Solution on potato cells?

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Introduction

Biology Investigation Title Investigating Osmosis Aim What is the effect of changing the concentration of Sodium Chloride Solution on potato cells? Prediction I predict that the lower the concentration of Sodium Chloride solution, the higher the final mass and length will be and the higher the concentration of Sodium Chloride solution, the lower the final mass and length. I also think that with a concentration of 0 and 1 molar, the change in mass and length will be greater than 0.125, 0.25 and 0.5 molar which I think will roughly stay the same. Osmosis is just a special case of diffusion. It is the net movement of water molecules across a semi-permeable membrane from a region of high water concentration to a region of low water concentration. In the diagram the water molecules diffuse into the concentrated sugar solution because the water molecules are small enough to pass through the membrane. The larger the sugar molecules are they are unable to pass through the membrane into the water solution. Osmosis will stop when the two solutions reach equal concentrations on either side of the membrane. ...read more.

Middle

Using a measuring cylinder measure 20ml of distilled water and pour into the test tube marked 0 molar. Prepare the Sodium Chloride solutions according to the table below. Concentration (molar) 1.0 0.5 0.25 0.125 Vol 2m NaCl (Gm�) 20cm� of 1.0m 10cm� of 0.1m 10cm� of 0.5m 10cm� of 0.25 Volume distilled water (cm�) 0 10 10 10 Into each previously marked test tube pour the correct solution and then place one chip in each test tube. Leave the test tube for twenty minutes. Remove the chips from the test tubes and dry with a paper towel. Re-measure the length and mass and record in a table. Repeat the experiment to obtain a second set of results. Results Set 1 Concentration (molar) Initial mass (g) Final mass (g) Change in mass (g) 0 0.8 0.9 +0.1 0.125 0.9 0.9 0 0.25 0.9 0.9 0 0.5 0.9 0.8 -0.1 1 0.9 0.7 -0.2 Concentration (molar) Initial length (mm) Final length (mm) Change in length (mm) 0 20 21 +1mm 0.125 20 20 0 0.25 20 20 0 0.5 20 19 -1mm 1 20 18 -2mm Set 2 Concentration (molar) ...read more.

Conclusion

My prediction was therefore correct. Evaluation My investigation in general worked very well. We obtained accurate results that we needed to draw a firm conclusion and all went according to the initial plan. There were no anomalous results in this investigation. I think that our method did work but it would have helped during the experiment if it was more detailed. I would think about rearranging some of the points and maybe doing some parts of the experiment differently or in a different order as we did seem a little unorganized throughout the experiment. If I was to do the experiment again I would label all of the test tubes before starting the experiment to avoid farther confusion. I do think we obtained enough results to draw a firm conclusion and the averages proved to produce an accurate graph for both the length and mass of the potato chip. I think our only concern would be that the length of the chip may not be perfectly accurate each time because of the measuring device used. Our results were all repeatable and followed the line of best fit. There were no anomalous results so I would therefore conclude that our experiment was a success and our results were reliable. ...read more.

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