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What is the effect of substrate concentration on the rate of reaction?

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Introduction

What is the effect of substrate concentration on the rate of reaction? Introduction: The rate of reaction is affected by the total number of enzyme and the substrate concentration. The rate of reaction tells us how fast a chemical reaction happens. In a rate of reaction the starting point is called Reactants and the finishing point is called products materials. There are three ways to measure the speed of a reaction. 1. Precipitation 2. Change in mass 3. Volume of gas given off. I did a preliminary experiment in chemistry and I worked with hydrochloric acid where I learned a lot of things about how to handle an experiment using gas syringe etc. the diagram below taken from: http://www.wpbschoolhouse.btinternet.co.uk/page03/3_31rates.htm#2 , it is the experiment I did and I found it easy to do using gas syringe so I think I could cope with my biology experiment. Like I said before there are three ways to measure the rate of reaction but I collected the gas given off by the reactants in chemistry because it is easy to collect them just by using gas syringe and also I know how to use a gas syringe which made me to do the experiment so easily. There are different sorts of reaction.1. Slowest reaction (e.g. rusting of iron) 2. Moderate speed reaction (e.g. metal reacting with acid) 3. Really fast reaction (e.g. explosion) Enzymes are biological catalysts. Enzymes are special protein that speeds up a reaction without being used up. ...read more.

Middle

So I will keep the Catalase Concentration constant in order to get accurate results. I will use the same size of potato by measuring it the ruler to see the size or electro scale or I might use baron to cut the potato. Hydrogen peroxide (H?O?) Change If I decrease the Hydrogen Peroxide there will be less collision which will make the rate of reaction to decrease so I should use the correct amount to increase the rate f reaction in order to get reliable results. I will create a solution by adding distilled water to hydrogen peroxide so I will monitor the hydrogen peroxide by pouring it onto the measuring cylinder and then I will do the same for the water. Apparatus: Apparatus Purpose Justification Hydrogen Peroxide When Hydrogen peroxide are broken down into products I will monitor the rate of reaction. This is a substrate. It is colourless and it is corrosive and irritant but the enzymes break it down to water and oxygen. Potato I will use this as a catalyse because it's job is to breakdown hydrogen peroxide. Potato is vegetable/ living thing so that means others can be used to react with hydrogen peroxide but potato is used as a catalyse in this experiment. Distilled water I will use this to make hydrogen peroxide in 5 different concentration. It is 100% pure water, that means it has purified by the distillation so it does not contain salts and other compounds some of the enzymes might change their PH of the solution if it's reacted with the distilled water. ...read more.

Conclusion

Improving planning: * I have to try to improve my reaction time because I should be able to everything by myself (e.g. when I add the potato in I should be able to place the bung as quickly as possible and then I should start the stop watch as quickly as I can because I have to do this to get accurate and reliable results because when I did my chemistry experiment I couldn't manage everything by myself and because of that I didn't get a reliable and accurate results. * I have to keep the syringe in my eye level because it will be easy for me to read it and I should be able to able to check the time every 5 minutes without doing something else that means I should concentrate in my experiment in order to get accurate results. * When I' am doing my experiment I should know what exactly I' am doing so that I don't have to waste my time and others by asking them how to do it so I should follow my method through out my experiment. * I will use conical flask instead of using beaker to do the experiment because conical flask has a small open on the top so it is easy to place the bung on to the conical flask but in beaker I can't put the bung which means the air releases outside so I won't get accurate and reliable results so it is way better to use conical flask. Sources that I used: http://www.wpbschoolhouse.btinternet.co.uk/page03/3_31rates.htm#2. http://www.blobs.org/science/enzyme/index.shtml#substrateconc www.tiscali.com http://www.purchon.com/biology/enzymes.htm http://www.courseworkhelp.co.uk/GCSE/Science/23.htm http://www.courseworkbank.co.uk/GCSE/Biology_Coursework/ http://www.s-cool.co.uk/topic_quicklearn.asp?loc=ql&topic_id=1&quicklearn_id=1&subject_id=17&ebt=26&ebn=&ebs=&ebl=&elc=4 http://www.gcsescience.com/rc.htm ?? ?? ?? ?? Biology Coursework ...read more.

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