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Enzyme amylase action on starch

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Introduction

Jack Chen 10KM Biology Assessed Practical: Enzyme amylase action on starch Aim: To find if the amount of starch present would affect the rate at which the amylase breaks it down. Hypothesis: The more starch there is, the longer it will take the amylase to break it down; the lesser starch there is, the faster it will take the amylase to break it down. Variables: Independent variable: The amount of starch Dependent variable: The time needed for amylase to breaks starch down. Constants: Same amount of amylase, and same temperature. Equipment: Starch suspension Amylase solution Syringes Iodine solution White tile Pipette Stop Watch Safety glasses Method: Put twenty very small drops of iodine solution, spaced well apart, onto a white tile. ...read more.

Middle

Record the color that the drop goes. Start a stopwatch as soon as possible. Continue to test one drop of the starch/amylase mixture every 20 seconds, and record the color the drop goes each time. Go on doing this until the iodine drop does not change color when adding the starch/amylase mixture to it. Repeat the experiment with different amount of starch for 15cm3 and 20cm3. Table of results: Amount of Amylase (cm3) Amount of Starch Suspension (cm3) Time for starch to break down (seconds) Syringe 1 5 10 120 Syringe 2 5 15 180 Syringe 3 5 20 240 Modification: A mixture of amylase/starch suspension is dropped every 20 seconds instead of 10 seconds because it might not be enough time to let the amylase to break down the starch in only 20 seconds. ...read more.

Conclusion

Conclusion: The results proved that my hypothesis was right. The more starch there is, the longer it will take the amylase to break it down; the lesser starch there is, the faster it will take the amylase to break it down. Evaluation: There are only one thing went wrong during the experiment. I did not job down the color change during every 20 seconds when starch is breaking down. Luckily, this was not a big deal because it did not affect the results. Everything else went quite well, there were just one or two odd results made. What I did with the odd results is I compared the odd results to the normal results and found out what was wrong, and then changed it. I could improve accuracy by doing more trials; unfortunately, I did not have much time to do more trials. ...read more.

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