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# Measuring Stomatal Numbers on Abaxial and Adaxial Surfaces

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Introduction

2nd EXPERIMENT: -

Measuring Stomatal Numbers on Abaxial and Adaxial Surfaces

Apparatus: -

*Microscope                                *Eight slides

*100 cm² Sheet of Acetate                *Eight cover slips

*Ball point marker pen                        *Scissors

*Fine forceps                                *0.3m ruler

## Method: -

1. Firstly use nail-varnish on the upper surface of two privet leaves which will be referred to as Upper-1 and Upper-2.
2. Carry out step 1 with a lower surface of another pair to be also labelled as Lower-1 and Lower-2.
3. Leave to dry.

Middle

Set-up the microscope and place Upper-1 underneath.Measure out using the 0.3m ruler and a ball point marker pen a square of 0.3cm by 0.3cm to give an area of 0.09cm². Cut outside the drawn border with a pair of scissors and situate into the eyepiece.Count the number of stomata within the marked space on the highest magnification possible and record the value.Undertake the same process with Upper-2, Lower-1 and Lower-2.Repeat the Method with four Laurel leaves.

Conclusion

(x cm²)

Where: –

x cm² = Area drawn on acetate = 0.09cm²

y = Number of stomata identified in 0.09cm² (either on upper or lower surface).

For step (d) of Method 1 the average stomatal numbers are calculated in a similar way: -

A)        (1cm²) _            ×     y      =      AVERAGE NUMBER OF STOMATA IN 1cm² AREA

(x cm²)                           (WHERE y  =  NO. OF STOMATA ON UPPER SURFACE)

B)        (1cm²)×     y      =      AVERAGE NUMBER OF STOMATA IN 1cm² AREA

(x cm²)                           (WHERE y  =  NO. OF STOMATA ON LOWER SURAFCE)

Therefore the total average number of stomata in 2 cm² area =  (A) + (B).

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