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  • Level: GCSE
  • Subject: Maths
  • Word count: 1542

Practical Report: Drosophila BreedingAim In this practical experiment I will cross wild fruit flies with vestigal fruit flies, to investigate which one is dominant or recessive and their characteristics

Extracts from this document...

Introduction

Hani Ali                           Page  of

BTEC ND1

Practical Report: Drosophila Breeding

Aim

In this practical experiment I will cross wild fruit flies with vestigal fruit flies, to investigate which one is dominant or recessive and their characteristics

Introduction

Drosophila melanogaster (fruit fly) is a common, generally innocuous insect that feeds on the fungi growing on fruit.

They are productive breeders, a single mating and they produce a hundreds of offspring and new generation can be bred every two weeks.

These characteristics make the fruit fly convenient organism for genetic studies and the fact that they have only four pairs of chromosomes, which are easily distinguishable with a light microscope. They have 3 pairs of autosome and one pair of sex chromosomes.

Female drosophila has a homologous pair of X chromosomes, and males have one X chromosome and one Y chromosome.

An embryologist named Thomas hunt Morgan, was the first to associate a specific gene with a specific chromosome, for his work he selected species of drosophila (fruit fly). Morgan spent a year breeding the flies and looking for variant individuals. Morgan discovered a single male fly with white eyes instead of usual red eye.

...read more.

Middle

XYBb

Male wild

XYBb

Male wild

Xb

XXBb

Female wild

XXBb

Female wild

XYBb

Female wild

XYBb

Male wild

Xb

XXBb

Female wild

XXBb

Female wild

XYBb

Male wild

XYBb

Male wild

Xb

XXBb

Female wild

XXBb

Female wild

XYBb

Male wild

XYBb

Male wild

                 

 Phenotype       = 100% wild     8 female : 8 male                

Observed result (cross 2)

Cross 2      wild female × vestigial male

F1 100% wild    18 male: 18 female

Expected result

XX = female

XY = male

BB = wild

Bb = wild

bb = vestigial

Cross 2

Parent     wild female × vestigial male

Genotype    XXBB           XYbb

Gametes   XB XB XB XB   Xb Xb Yb Yb

F1 generation

                                                              Male gametes

Xb

Xb

Yb

Yb

XB

XXBb

Female wild

XXBb

Female wild

XYBb

Male wild

XYBb

Male wild

XB

XXBb

Female wild

XXBb

Female wild

XYBb

Male wild

XYBb

Male wild

XB

XXBb

Female wild

XXBb

Female wild

XYBB

Male wild

XYBb

Female wild

XB

XXBb

Female wild

XXBb

Female wild

XYBb

Male wild

XYBb

Male wild

Phenotype = 100% wild          8 male: 8 female

Observed result (cross 3)

Cross 3F1 self cross from cross 1

             Wild male × vestigial female

F 2     18 wild   9 male : 9 female

          6 vestigial  3 male : 3 female

Observed ratio = 3:1

Expected results

XX = female

XY = male

...read more.

Conclusion

Sources of error

  • There could be less pairing number, because Flies could be killed by overdose of flynap
  • Not enough yeast used ( the flies grown eat only yeast given, but there was not enough yeast they may die.
  • There could be human error by using the equipments and flies may not be identified properly (males from females)
  • May be not all females are virgin and if so it wouldn’t be a pure-breeding

...read more.

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