A INVESTIGATION TO MEASURE THE EFFECT OF DIFFERENT ANTI-MICROBIAL COMPOUNDS ON BACTERIAL GROWTH
PLAN
Aim
The aim of this investigation is to see whether the different anti-microbial substances effects the growth of bacteria at different dilution's.
Prediction
All the anti-microbial solutions will create large clearance zones at the undiluted level, but the clearance zones will decrease as the solution becomes more diluted.
Variables
Incubation temperatures at which the experiments are done are kept constant.
The same bacterial culture is used.
The same nutrient agar is used.
INTRODUCTION
Variables
Incubation temperatures at which the experiments are done are kept constant.
The same bacterial culture is used.
The same nutrient agar is used.
Apparatus
Bacterial Culture
Bunsen Burner
Heat Proof Mat
Tweezers
6 Sterile Petri dishes
6 x 15cm3 of molten nutrient agar
Three different antiseptics (1)Dettol (2)TCP (3)Savlon
Three different disinfectants (1)Dettox (2)Domestos (3)Vortex
Sterile water for dilution
8 Sterile Universal Bottles
24 x 0.5cm diameter filter paper disks.
Background
About 200 species of bacteria are pathogenic, or disease causing, for humans. Pathogenicity varies widely among various species and is dependent on both the virulence of the particular species and the condition of the host organism. Among the more invasive bacteria responsible for human disease are those that cause cholera, lockjaw, gas gangrene, leprosy, plague, bacillary dysentery, tuberculosis, syphilis, typhoid fever, diphtheria, undulant fever, and several forms of pneumonia. Until the discovery of viruses, bacteria were considered the causative agents of all infectious diseases.
PLAN
Aim
The aim of this investigation is to see whether the different anti-microbial substances effects the growth of bacteria at different dilution's.
Prediction
All the anti-microbial solutions will create large clearance zones at the undiluted level, but the clearance zones will decrease as the solution becomes more diluted.
Variables
Incubation temperatures at which the experiments are done are kept constant.
The same bacterial culture is used.
The same nutrient agar is used.
INTRODUCTION
Variables
Incubation temperatures at which the experiments are done are kept constant.
The same bacterial culture is used.
The same nutrient agar is used.
Apparatus
Bacterial Culture
Bunsen Burner
Heat Proof Mat
Tweezers
6 Sterile Petri dishes
6 x 15cm3 of molten nutrient agar
Three different antiseptics (1)Dettol (2)TCP (3)Savlon
Three different disinfectants (1)Dettox (2)Domestos (3)Vortex
Sterile water for dilution
8 Sterile Universal Bottles
24 x 0.5cm diameter filter paper disks.
Background
About 200 species of bacteria are pathogenic, or disease causing, for humans. Pathogenicity varies widely among various species and is dependent on both the virulence of the particular species and the condition of the host organism. Among the more invasive bacteria responsible for human disease are those that cause cholera, lockjaw, gas gangrene, leprosy, plague, bacillary dysentery, tuberculosis, syphilis, typhoid fever, diphtheria, undulant fever, and several forms of pneumonia. Until the discovery of viruses, bacteria were considered the causative agents of all infectious diseases.