A Investigation to measure the effect of different anti-microbial compounds on bacterial Growth

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A INVESTIGATION TO MEASURE THE EFFECT OF DIFFERENT ANTI-MICROBIAL COMPOUNDS ON BACTERIAL GROWTH

PLAN

Aim

The aim of this investigation is to see whether the different anti-microbial substances effects the growth of bacteria at different dilution's.

Prediction

All the anti-microbial solutions will create large clearance zones at the undiluted level, but the clearance zones will decrease as the solution becomes more diluted.

Variables

Incubation temperatures at which the experiments are done are kept constant.

The same bacterial culture is used.

The same nutrient agar is used.

INTRODUCTION

Variables

Incubation temperatures at which the experiments are done are kept constant.

The same bacterial culture is used.

The same nutrient agar is used.

Apparatus

Bacterial Culture

Bunsen Burner

Heat Proof Mat

Tweezers

6 Sterile Petri dishes

6 x 15cm3 of molten nutrient agar

Three different antiseptics (1)Dettol (2)TCP (3)Savlon

Three different disinfectants (1)Dettox (2)Domestos (3)Vortex

Sterile water for dilution

8 Sterile Universal Bottles

24 x 0.5cm diameter filter paper disks.

Background

About 200 species of bacteria are pathogenic, or disease causing, for humans. Pathogenicity varies widely among various species and is dependent on both the virulence of the particular species and the condition of the host organism. Among the more invasive bacteria responsible for human disease are those that cause cholera, lockjaw, gas gangrene, leprosy, plague, bacillary dysentery, tuberculosis, syphilis, typhoid fever, diphtheria, undulant fever, and several forms of pneumonia. Until the discovery of viruses, bacteria were considered the causative agents of all infectious diseases.
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Antiseptics are the, physical or chemical agents that prevent putrefaction, infection, and analogous changes in food and living tissue by destroying or arresting the development of micro-organisms. The practice of using antiseptics in the care and treatment of wounds was begun by the English surgeon Joseph Lister in 1868.

METHOD

The work surface was sterilised using a disinfectant. The apparatus should be autoclaved at 121oC and at high pressure to kill all present bacteria. A sterile screw cap bottle containing 15cm3 of the molten agar should be used. Before the agar solidifies 15cm3 of bacteria should ...

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