AIM: To determine the concentration of ascorbic acid in orange juices

Authors Avatar by swissami (student)

Title: Ascorbic Acid Test

Aim: To determine the concentration of ascorbic acid in orange juices

Introduction: Ascorbic acid is a reductone sugar acid with antioxidant properties, i.e., it is capable of inhibiting the oxidation of other molecules. It is one form of vitamin C and is therefore an essential nutrient for human species and certain other animal species. In animals, it plays a role in wound-healing and in preventing bleeding from capillaries and in humans, it is essential to a healthy diet and to the maintenance of connective tissue.  The major food sources of ascorbic acid are green vegetables and citrus foods.

Materials/ Apparatus:

0.1% DCPIP

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4.0mg/ml of ascorbic acid

2.0 mg/ml of ascorbic acid

1.0mg/ml of ascorbic acid

0.5mg/ml of ascorbic acid

Orange juice

Sour orange juice

Lemon juice

Test-tubes

Test-tube rack

Procedure:

1.        A small syringe was used to add 1ml of DCPIP to a test tube and the test tube was put to stand.

2.        A 1ml syringe was filled with 4.0mg/ml of ascorbic acid solution. The solution was then added drop by drop to the DCPIP solution and stirred gently with a glass rod.

3.        The number of drops taken to decolorize the DCPIP solution was recorded.

4.        The procedure was ...

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