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Amylase activity in different temperatures

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Aim To investigate the activity of the enzyme amylase at different temperatures. Introduction Amylase is a digestive enzyme found in the salivary glands and the pancreas. Enzymes speed up biochemical reactions by lowering the activation energy required to break bonds, they are known as 'biological catalysts'. Enzymes are globular proteins and have tertiary or quaternary structure. Enzymes are 'specific proteins' due to the shape of the active site (the site where the substrate attaches), meaning they will only break down specific substrates (in this case starch). Amylase breaks down the insoluble polysaccharide (starch) into a disaccharide (maltose) by hydrolysis. Enzymes work more efficiently at different pH and temperatures. ...read more.


The graph shows a steep rise in absorbance between 4oC and 40oC, this shows that as the temperature increased so did the rate of hydrolysis of starch into maltose. As the temperature increases the molecules vibrate more due to more kinetic energy, this speeds up the activity of the amylase as the substrates move faster and there is a higher chance of successful collisions. The graph shows that between 50oC to 70oC there is a steep decrease in absorbance meaning there has been fewer hydrolysis of starch. The decrease in absorbance means that the amylase enzyme became less active. Enzymes can only withstand certain temperatures, the graph shows that the optimum temperature of amylase is 40oC as that is the temperature where the hydrolysis of starch to maltose was the fastest. ...read more.


Some of the errors or limitations were due to the apparatus and some are human errors. The experiment can be more reliable if each there were 3 mixtures used in separate boiling tubes for each temperature. An average can be taken from the 3 results for each temperature making the experiment more reliable. The methods shows to do the experiment with all the different temperatures with a 30second time gap. This can make the experiment unreliable as the boiling tubes can be placed a little earlier or later than the allocated 30 seconds. More reliability of the experiment can be maintained if each different temperature is done after the previous has finished, this may take longer but will give more reliable results. ?? ?? ?? ?? ...read more.

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