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An Experiment to find out the Effect of Temperature on the Enzyme Catalase.

Extracts from this document...

Introduction

An Experiment to find out the Effect of Temperature on the Enzyme Catalase Variable How Will it be controlled? Why will it be controlled? Catalase I will measure out carefully 0.3cm3 using a 1cm3 micro pipette It will keep the same volume of enzyme in solution in order for the results to be accurate and valid, because if there is more catalase the reaction will occur faster as more enzyme substrate complexes will be formed. Hydrogen Peroxide I will measure out carefully 5cm3 using a 5cm3 graduated pipette It will keep the same volume of the substrate in solution in order for the results to be accurate and valid, because if there is more Hydrogen Peroxide more oxygen will be emitted and an enzyme substrate complex will be formed more often. pH Buffer I will measure out carefully 0.5cm3 using a 1cm3 micro pipette It will keep the pH levels constant in the solution so that the results are accurate and valid, because if there are more hydrogen ions or hydroxide ions it may affect the rate at which the catalase functions. Light Intensity I will keep the Hydrogen Peroxide in a brown jar It will keep it from breaking down on its own, as if it did the results I obtain would be false and inaccurate. Temperature I will measure it using a Thermometer So that the effect of different temperatures can be investigated Apparatus * Hydrogen Peroxide-This is the substrate of catalase * pH buffers 6,7,8-So that the pH levels can be kept constant * Clamp Stands-To hold the boiling tubes and the gas burette * Gas Burette-It is an accurate way to measure oxygen * Tub of water-To prevent the gas burette from emptying * Boiling Tubes-To carry out the reaction in * Delivery Tube-To transport the oxygen from the reaction to the gas burette * Safety Goggles-To protect my eyes from the Hydrogen Peroxide * Glowing Splint-To test for oxygen in the gas burette * Graduated Pipette-To measure out ...read more.

Middle

I controlled the pH by adding 0.5cm3 pH 8 buffer to every reaction so that hydrogen ions or hydroxide ions were kept constant because they may affect the rate at which the catalase functions. I controlled the volume of catalase each time because if there is more catalase in a specific reaction than in the other reactions it will occur faster as more enzyme substrate complexes will be formed. I controlled the volume of the Hydrogen Peroxide each time because if there is more Hydrogen Peroxide in a particular experiment more oxygen will be emitted, because enzyme substrate complexes will be formed more often. I controlled the light intensity by keeping the Hydrogen peroxide in a brown jar and conducting the entire experiment in the same room. If the light intensity was varied it may have forced the Hydrogen Peroxide to break down faster. Results Preliminary experiment results to work out optimum pH around neutral conditions, at room temperature: ph 6 Time in seconds 1st attempt Oxygen released (cm3) 2nd attempt Oxygen released (cm3) 3rd attempt Oxygen released (cm3) Average Value (cm3) 5 4 0.5 1 1.8 10 10.5 9 14.5 11.3 15 13 11 16 13.3 20 16 12.5 20 16.16 25 19 15 27 20.3 30 25 17 32 24.6 35 34 21 38 31 40 40 27 45 37.3 ph7 Time in seconds 1st attempt Oxygen released (cm3) 2nd attempt Oxygen released (cm3) 3rd attempt Oxygen released (cm3) Average Value (cm3) 5 1.5 3 7.5 4 10 10 13 15 12.6 15 12 16 18 15.5 20 15 19 23 19 25 20.5 24.5 29.5 24.83 30 27 30 39 32 35 35 36 47 39.3 40 41 43 50 44.6 ph8 Time in seconds 1st attempt Oxygen released (cm3) 2nd attempt Oxygen released (cm3) 3rd attempt Oxygen released (cm3) Average Value (cm3) 5 9 12.5 6 9.16 10 19 18 15 17.3 15 25 21 21 22.3 20 29 26 23.5 26.16 25 37 32 28 32.3 30 42 38 ...read more.

Conclusion

This had an affect on the earlier points on my graphs, but not on the general pattern and hence would not have affected any conclusions that I have drawn. If I did this experiment in the future I might unhook the delivery tube each time I wanted to measure a reading from the gas burette. This would help as I would have more accurate readings by creating more time to collect the measurements. I would also use a more accurate syringe to measure out the Hydrogen Peroxide so that the same amount of substrate was added to each reaction, thus giving me more reliable results. It would also help to support the conclusions that I have drawn from this experiment. The evidence I collected is what I expected to find, however in my opinion it is not enough to produce a firm valid conclusion. The maximum yield of oxygen at 35�C is roughly normal although I do expect to find that the optimum would be a little higher, than the max yield that I obtained. In order to produce a firm valid conclusion I would have to repeat the experiment on the temperatures between 35�C-45�C, so that I could find out the exact optimum temperature. Also I would have to conduct the experiment at lower temperatures, on the rising curve, so that I can obtain a full reaction rate curve. I could have also measured the pH in every reaction instead of just relying on the pH buffer to do its job. This would have further validated the results I obtained. ?? ?? ?? ?? This document was downloaded from Coursework.Info - The UK's Coursework Database - http://www.coursework.info/ This document was downloaded from Coursework.Info - The UK's Coursework Database - http://www.coursework.info/ This document was downloaded from Coursework.Info - The UK's Coursework Database - http://www.coursework.info/ This document was downloaded from Coursework.Info - The UK's Coursework Database - http://www.coursework.info/ ...read more.

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