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An Experiment to Investigate the Effectof the Enzyme Catalase on Hydrogen Peroxide when the Temperature is Changed.

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Introduction

An Experiment to Investigate the Effect of the Enzyme Catalase on Hydrogen Peroxide when the Temperature is Changed. Plan: Chemical reactions take place in all living cells. All of these reactions are enzyme-driven. Some of these reactions produce by-products. One by-product is Hydrogen Peroxide (H2O2). It is a poison and must be removed from the cells. The cell produces an enzyme called Catalase to break down the Hydrogen Peroxide into water and oxygen. This experiment will investigate the effect of temperature on the rate of the breaking down of Hydrogen Peroxide by Catalase. I will do this by measuring how much oxygen is produced when Catalase and Hydrogen Peroxide react. I predict that as the temperature increases, the rate of the reaction will increase. I think that when the temperature is doubled, so the reaction rate will also double. This is due to the particle theory. As the temperature rises, the particles move faster and collide more often. This gives the "lock and key" reaction more chance to occur. ...read more.

Middle

The apparatus will be set out as shown below: Preliminary Work I did a preliminary experiment to give me an idea as to which values and range I should use for my temperatures, and to check if the equipment and setup I had chosen was suitable for the experiment. Here are the results of that experiment: Temperature (�C) Oxygen Produced (cm3) 30 2.7 40 2.4 50 4.5 60 2.8 70 1.4 These results are not ideal, as they have only been done once so no averages can be found to improve accuracy, but they give me an idea as to which values to use as my temperatures. It does show however that Catalase seems to start denaturing at around 60�C, and that it appears to work best at around 50�C. Table Of Results For Main Experiment Temperature (in �C) Start Level on burette End Level on burette Oxygen Produced (cm3) Average of Expt. 1+2 (cm3) 2.5 Expt.1 47.1 44.3 2.8 2.3 2.5 Expt.2 46.4 44.6 1.8 ------------- Room Expt.1 46.3 41.5 4.8 4.65 Room Expt.2 45.3 40.8 4.5 ------------- 41 Expt.1 40.4 35.0 5.4 5.45 41 Expt.2 41.0 35.5 ...read more.

Conclusion

This cannot really be helped unless there was a way of keeping the concentration the same all the way through the whole of the experiment, maybe by adding more potato as it starts to run out. The equipment could be improved. A syringe with a needle may be used to inject the H2O2 through the bung directly into the boiling so that there is no lag time for the oxygen to escape while the bung is being put on the tube. I think the evidence I have is strong enough to support the conclusion "as the temperature rises, the rate of reaction increases except where the enzyme is denatured," but not strong enough to give a quantitative result. This could be achieved by using more sophisticated equipment so that no oxygen was lost at all, and testing it several times instead of just two or three times. More evidence could be acquired by carrying out more experiments. This could be to get a more accurate reading of points not tested here, such as 55�C or 15�C (these may be read off the best fit line on the graph, but would not be as accurate as actually testing it). Gareth Davies 11PI S3 ...read more.

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