An experiment to investigate the rate of anaerobic respiration of yeast in various respiratory substrates

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Practical Method

Title

"An experiment to investigate the rate of anaerobic respiration of yeast in various respiratory substrates."

In this experiment, the independent variable is various different respiratory substrates being used (glucose, sucrose, maltose, lactose) and the dependent variable is the rate of respiration (measured by movement of manometer fluid which moves in relation to the amount of carbon dioxide released).

Apparatus

Yeast

Glucose

Sucrose

Maltose

Lactose

pH7 buffer

Top pan balance

Stopwatch

Thermometer

Manometer fluid

Capillary tube

20ml & 1ml syringe

100ml beakers

Distilled water

Water trough

Kettle

Stirring rod

Stopwatch

Spatula

Background information

My investigation will involve analysing how yeast respires in various different substrates: Glucose, Lactose, Maltose and Sucrose. All four of these respiratory substrates are carbohydrates.

Glucose

Glucose is a monosaccharide sugar, which is a 'simple sugar' that have between 3 and 10 carbon atoms per molecule. They are sweet and all soluble in H2O. It has the chemical composition C6H12O6. Glucose is a white crystalline solid but is less sweet then ordinary table sugar. Powdered dry glucose