• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

An experiment to investigate whether a change in the concentration of hydrogen peroxide substrate will affect the rate of Oxygen production.

Extracts from this document...


An experiment to investigate whether a change in the concentration of hydrogen peroxide substrate will affect the rate of Oxygen production. Background In this experiment I will be investigating whether using a different concentration of substrate (hydrogen peroxide) will affect the rate at which oxygen is produced. The reaction shown in this experiment represents the reaction that occurs in the body that turns the substrate (the reactant) in to the oxygen and water (the product). Enzymes are proteins that work as biological catalysts, meaning they speed up reactions in the cell. The Enzyme works by joining two substrates, or splitting one, this joining and splitting occurs in the active site. The active site is the section of the enzyme that is a specific shape which fits certain substrate molecules. They are said to fit like "a lock and key." Whilst the substrate is connected to the enzyme, the enzyme weakens some of the bonds in the substrate. This either encourages the substrate to split into component elements or to join substrates, leading to the formation of a product. The product is released from the active site and the enzyme can repeat this process again. Hydrogen peroxide enzyme Oxygen+Hydrogen The aim of this experiment was to find out if a change in the concentration of the substrate (hydrogen peroxide) ...read more.


8. Pour the distilled water into the boiling tube and cork it with the other end of the delivery tube, then start the stop watch. 9. After five seconds measure the amount of oxygen produced on the measuring cylinder. 10. Measure the amount of oxygen after 10, 15 and 20 seconds. 11. Repeat steps 1-10 twice then take an average 12. Repeat steps 1-11 with 5%, 10%, 15%, and 20% concentration of Hydrogen peroxide. Results Concentration of hydrogen peroxide (%) Time taken (s) Oxygen produced (ml) Average (ml) Attempt 1 Attempt 2 Attempt 3 Attempt 4 0 5 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 30 0.0 0.0 0.0 0.0 5 5 3.0 2.0 1.0 2.0 10 5.0 5.0 2.0 4.0 15 7.0 7.0 4.0 6.0 20 8.0 8.0 5.0 7.0 25 9.0 9.0 5.0 7.7 30 9.0 9.0 7.0 8.3 10 5 6.0 5.0 4.0 5.0 10 10.0 12 9.0 10.3 15 14.0 15 14.0 14.3 20 16.0 15.0 17.0 16.0 25 16.0 16.0 18.0 16.7 30 16.0 16.0 19.0 17.0 15 5 5.0 10.0 7.00 7.0 6.3 10 11.0 15.0 11.0 12.0 11.3 15 14.0 16.0 14.0 14.0 14.0 20 16.0 18.0 14.0 15.0 15.0 25 16.0 19.0 14.0 16.0 15.3 30 16.0 20.0 15.0 16.0 15.7 20 5 7.0 10.0 11.0 9.3 10 10.0 15.0 19.0 17.7 15 ...read more.


There were some problems with the experiment, like the anomalous result for 15%. There are many things that could have caused them, like human error, The person operating the stopwatch could have had a delayed reaction time, the person reading the results could have not read them completely correctly; also the temperature of the room could have varied on different days, varying the enzyme activity; and we were unsure how to control the ph levels which could have varied ever so slightly, varying the results. If I had a chance to redo this experiment I would change lots of things to make the investigation fairer. Firstly instead of taking the measurements three times then taking an average, I would take the measurements five times then take an average to reduce the effect of outliers. Secondly I would make sure to monitor and control the temperature more closely to ensure that the test is fair, because if the temperature has increased the enzymes gain energy meaning that they would either have an increased activity or they will denature, changing the result of the experiment. Also I would have a longer period of time to measure the result in. Instead of every five seconds for thirty seconds, every five seconds for a minute so we could also compare when the different concentrations stop reacting, this could also change the outcome of the 10% and 15% results because the 15% might cross back over the ten percent making my prediction correct and the investigation a success. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Life Processes & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Life Processes & Cells essays

  1. Free essay

    Investigation Into the Effect of Substrate Concentration On The Activity Of An Enzyme

    * Stop Watch: we will need a stopwatch to record how fast reaction takes. * Syringes: We will use two syringes one syringe to transport the Hydrogen Peroxide and the other to transport the water so that the Hydrogen Peroxide is not diluted in water before it is added to the Catalase this will make the test fairer.

  2. How Does Changing the Substrate Concentration Affect the Rate of an Enzyme Controlled Reaction?

    I will make my investigation a fair test by repeating it more than once; I will use timings of 20 seconds, and will repeat this for each concentration of substrate 3 times and calculate an average. Another thing I will do to make my investigation a fair test is by using the same amount of enzyme(1 spatula)

  1. How will Concentration affect the Volume and Rate of Oxygen Released when reacting Hydrogen ...

    If there are more substrate for the enzyme to react with then the rate will be faster, because there is more chance for a successful collision if there is an increase of the substrate into a fixed amount of enzyme.

  2. 'The effect of changing the concentration of the substrate hydrogen peroxide on the rate ...

    Temperature of hydrogen peroxide The temperature of hydrogen peroxide can affect the rate of an enzyme-controlled reaction because below the optimum temperature, as the temperature rises, so does the rate of activity gradually. This is because increasing the temperature increases the kinetic energy of the substrate and the enzyme.

  1. The aim of this experiment is to demonstrate that the substrate Hydrogen Peroxide will ...

    In an enzyme catalysed reaction, such as the decomposition of Hydrogen Peroxide, this increases the rate at which the enzyme and substrate molecules meet and therefore the rate at which the products are formed. The temperature, at which enzymes work most efficiently, is known as the Optimum Temperature.

  2. The activity of Enzyme Concentration on Hydrogen Peroxide.

    The reaction process is as follows: 2H202 2H20 + O2 This Catalase-mediated reaction is extremely important in the cell because it prevents the accumulation of hydrogen peroxide, a strong oxidizing agent that tends to disrupt the delicate balance of cell chemistry.

  1. Does changing the concentration of enzymes affect the rate of an enzyme- controlled reaction?

    I then stopped the timer at three minuets and recorded my findings. I proceed to do this same process three times, for each amount of potato cubes changing the hydrogen peroxide between. No. of potato cubes Number of bubbles in 3 minutes Average Experiment 1 Experiment 2 Experiment 2 1

  2. To investigate whether the concentration of substrate (starch) affects the time taken for color ...

    Aim: My investigation?s aim is to find out, how different concentrations of enzymes affect reaction times. This will allow me to notice any difference in reaction times, also it will help me find the best concentration to work at. I chose to do concentrations as maintaining the temperature of a

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work