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An experiment to investigate whether a change in the concentration of hydrogen peroxide substrate will affect the rate of Oxygen production.

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An experiment to investigate whether a change in the concentration of hydrogen peroxide substrate will affect the rate of Oxygen production. Background In this experiment I will be investigating whether using a different concentration of substrate (hydrogen peroxide) will affect the rate at which oxygen is produced. The reaction shown in this experiment represents the reaction that occurs in the body that turns the substrate (the reactant) in to the oxygen and water (the product). Enzymes are proteins that work as biological catalysts, meaning they speed up reactions in the cell. The Enzyme works by joining two substrates, or splitting one, this joining and splitting occurs in the active site. The active site is the section of the enzyme that is a specific shape which fits certain substrate molecules. They are said to fit like "a lock and key." Whilst the substrate is connected to the enzyme, the enzyme weakens some of the bonds in the substrate. This either encourages the substrate to split into component elements or to join substrates, leading to the formation of a product. The product is released from the active site and the enzyme can repeat this process again. Hydrogen peroxide enzyme Oxygen+Hydrogen The aim of this experiment was to find out if a change in the concentration of the substrate (hydrogen peroxide) ...read more.


8. Pour the distilled water into the boiling tube and cork it with the other end of the delivery tube, then start the stop watch. 9. After five seconds measure the amount of oxygen produced on the measuring cylinder. 10. Measure the amount of oxygen after 10, 15 and 20 seconds. 11. Repeat steps 1-10 twice then take an average 12. Repeat steps 1-11 with 5%, 10%, 15%, and 20% concentration of Hydrogen peroxide. Results Concentration of hydrogen peroxide (%) Time taken (s) Oxygen produced (ml) Average (ml) Attempt 1 Attempt 2 Attempt 3 Attempt 4 0 5 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 30 0.0 0.0 0.0 0.0 5 5 3.0 2.0 1.0 2.0 10 5.0 5.0 2.0 4.0 15 7.0 7.0 4.0 6.0 20 8.0 8.0 5.0 7.0 25 9.0 9.0 5.0 7.7 30 9.0 9.0 7.0 8.3 10 5 6.0 5.0 4.0 5.0 10 10.0 12 9.0 10.3 15 14.0 15 14.0 14.3 20 16.0 15.0 17.0 16.0 25 16.0 16.0 18.0 16.7 30 16.0 16.0 19.0 17.0 15 5 5.0 10.0 7.00 7.0 6.3 10 11.0 15.0 11.0 12.0 11.3 15 14.0 16.0 14.0 14.0 14.0 20 16.0 18.0 14.0 15.0 15.0 25 16.0 19.0 14.0 16.0 15.3 30 16.0 20.0 15.0 16.0 15.7 20 5 7.0 10.0 11.0 9.3 10 10.0 15.0 19.0 17.7 15 ...read more.


There were some problems with the experiment, like the anomalous result for 15%. There are many things that could have caused them, like human error, The person operating the stopwatch could have had a delayed reaction time, the person reading the results could have not read them completely correctly; also the temperature of the room could have varied on different days, varying the enzyme activity; and we were unsure how to control the ph levels which could have varied ever so slightly, varying the results. If I had a chance to redo this experiment I would change lots of things to make the investigation fairer. Firstly instead of taking the measurements three times then taking an average, I would take the measurements five times then take an average to reduce the effect of outliers. Secondly I would make sure to monitor and control the temperature more closely to ensure that the test is fair, because if the temperature has increased the enzymes gain energy meaning that they would either have an increased activity or they will denature, changing the result of the experiment. Also I would have a longer period of time to measure the result in. Instead of every five seconds for thirty seconds, every five seconds for a minute so we could also compare when the different concentrations stop reacting, this could also change the outcome of the 10% and 15% results because the 15% might cross back over the ten percent making my prediction correct and the investigation a success. ...read more.

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