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An Experiment to show the affect of Copper Sulphate Solution on Catalase.

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Introduction

An Experiment to show the affect of Copper Sulphate Solution on Catalase. Introduction. Reactions of chemicals are an important part of life. Enzymes are biological catalysts. A catalyst is a substance which increases the rate of a reaction by attracting the reactants, this makes them more likely to collide and react. There are other conditions which can effect the rate of a reaction (these are mention in the prediction). Aim. The aim of this experiment is to discover the effect that copper sulphate has of the enzyme catalase. This will be achieved by using hydrogen peroxide, catalase/potato (rich in catalase) and a buffer. The effect will be the amount of oxygen gas that is released which is measured using a graduated gas syringe. Hypotheses. Hypothesis: Copper Sulphate solution will inhibit (slow down) the rate of the reaction between Hydrogen peroxide and catalase. The concentration of the Copper sulphate solution is inversely proportional to the rate of the reaction. Null Hypothesis: Copper Sulphate solution has no effect of the rate of reaction between Hydrogen Peroxide and catalase. As the Concentration of the copper sulphate solution is altered the rate of reaction will remain constant. To prove one of these hypotheses a fair test experiment will have to be strictly obeyed to ensure a true representation. Prediction. An increase in the concentration of the copper sulphate solution is inversely proportional to the rate of the reaction. High Concentration = Slow Rate of reaction etc. I predict this because copper sulphate solution contains the heavy metal ion Copper. ...read more.

Middle

Diagrams to Show the process of inhibition Variables. * Presence of an inhibitor - If the copper sulphate solution contains an inhibitor, the rate of the reaction would be slow. This can only be proven if the variable are kept constant (apart from the concentration of the copper sulphate solution). The indication of the copper sulphate solution being an inhibitor will be confirmed by the comparison of the results with the results of the 'standard test'. If a sign of inhibition is also shown in the standard test this will prove that it is not the copper sulphate solution which inhibits the reaction of hydrogen peroxide and catalase. Another variable would have to be altered to discover the inhibitor of the reaction. * pH - This is controlled by the volume of the buffer and it's pH. The pH of the solution will be tested before each experiment using a pH meter. The pH should measure roughly 7, as this is the optimum pH of catalase (and the majority of enzymes). This variable must be kept constant in order to abide by the fair test criteria. * Temperature - A water bath can be used to maintain a constant temperature throughout the reaction. The reactant are put in the water bath for a minute to achieve equilibrium. The Conical flask should not be hand held. This would increase the temperature inside the conical flask which would cause the oxygen gas to expand and move at a quicker rate into the gas syringe. ...read more.

Conclusion

Control Experiment. Put 5cm� of the buffer (pH 7) in a conical flask with 2cm� of Hydrogen peroxide and water. The control is set up to test if the rate of the reaction is dependent on the concentration of the copper sulphate solution. In the copper sulphate solution inhibits the reaction this control should have a faster rate of reaction. This will prove whether the presence of copper sulphate solution is the inhibitor or if it is another substance within the reaction i.e. Hydrogen Peroxide. Method with Copper Sulphate Solution. The apparatus was set up as shown in the diagram above. They were placed in a water bath of temperature 30�C and left to equilibrilate. 5cm� of the buffer (pH 7) was placed in a conical flask with 2cm� of hydrogen peroxide. 1g of catalase powder/potato sample was added to the conical flask. The volume of gas produced by the reaction was measured using a graduated gas syringe (which was connect to the conical flask using a delivery tube). The volume of gas was recorded in a table (shown in the results section). NOTE: The hydrogen peroxide was placed in the syringe and added to the mixture when all bungs had been placed on. This allows all gas from the start of the reaction to be collected. The conical flask is then gentle swirled to mix the reactants. The procedure was repeated but with adding copper sulphate solution of amounts: 2cm�, 4cm�, 6cm�, 8cm�,& 10cm� in different test tubes. All results were recorded using a table which can be found in the results section. ...read more.

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