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An experiment to show the effect of hydrogen peroxide on the catalase.

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Introduction

An experiment to show the effect of hydrogen peroxide on the catalase Aim An investigation to see the volume of oxygen produced when hydrogen peroxide was added to the catalase Method * The equipment was set up * With a cork borer several tubes from the potato were cut out * Using a ruler, 3mm long pieces of potato were cut up * The pieces of potato were put into the round bottomed flask * With the tap closed, 10cm� of hydrogen peroxide was poured into the reservoir * The tap was opened until all the hydrogen peroxide had poured into the round-bottomed flask and then it was closed firmly. * The pipette was put into the test tube of water * The timer was started for 3 minutes * The amount of bubbles were counted, using a calculator * After 3 minutes the number of bubbles were recorded * The round bottomed flask was washed out with distilled water * The experiment was repeated 3 more times Results table Bubbles ...read more.

Middle

It is used for removing Hydrogen Peroxide from the cells. Hydrogen Peroxide is the poisonous by-product of metabolism. Catalase speeds up the decomposition of Hydrogen Peroxide into water and oxygen. Each enzyme has an active site which a substrate must bind to form an enzyme-substrate complex with. This is what causes the reaction and the products to be produced. In this experiment the catalase was the enzyme and the hydrogen peroxide was the substrate. The oxygen was the product formed after the enzyme-substrate complexes were formed. This reaction that took place was: As the potato pieces increased, so did the volume of gas released. This is because more enzymes became available for the substrates to bind with. When the number of potato pieces reach 60, there is less oxygen being produced, this is because there are too many enzymes but not enough substrates. These substrates all get used up and after a certain time there are very little substrates left. ...read more.

Conclusion

It may have diluted the experiment and caused less enzyme-substrate complex's to be formed. The quick fit equipment may not have been a tight fit and so some gas could have been released from it. When the hydrogen peroxide was added to the potato, some gas may have been lost through the pipette before it was put into the water. To help make this experiment more accurate, it was repeated four times and then the averages of the results were used to plot a graph. I tried to keep all the variables the same for all the experiments. However, in reality it is impossible to keep all the variables precisely the same. The variables such as temperature and PH were unable to be kept constant as the required equipment was unavailable. I think the range used was fine, it was a wide enough, ranging from 5 to 60 potato pieces. However, if I were to repeat the experiment I would insert some more in-between 50 and 60 pieces so it can be observed at exactly what point the number of oxygen bubbles released reduces. Harpreet Sekhon ...read more.

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