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An experiment to show the lowest concentration of copper (II) sulphate solution needed to bring about full denaturation of egg albumen.

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An experiment to show the lowest concentration of copper (II) sulphate solution needed to bring about full denaturation of egg albumen Introduction Egg albumen is a complicated protein that is more commonly know as egg white. This protein becomes denatured when copper (II) sulphate is added at a certain concentration. This is because the cations from the copper ions react with the amino acids carboxylate anions which form peptide bonds. This in turn affects the secondary structure of the egg albumen since there is a disruption in the peptide bonds and the protein reforms as a fibrous protein precipitating out of the solution and becoming opaque, the copper ions also react with the hydrogen in the amine group causing the primary structure to fail also. ...read more.


It can also be used to clean other apparatus. Egg albumen this is needed to perform the experiment 0.1 mol dm-3 copper (II) Sulphate solution I will use this to make all my concentrations, e.g. 0.05, 0.025 etc Test tubes Test tube rack Pipettes and syringes these will be used to measure certain amounts accurately Preliminary work From looking at the results I have gathered. It showed me the importance of the range of concentrations used. My results were reasonable I know this because they were similar 2 most of my class mates This also helped me narrow down the range of result I would take I decided that I would only take between0.005 and 0.00625 Prediction I predict that it ...read more.


3) Now add 5 cm3 of egg albumen to each test tube shake to mix and then wait for 5 minutes 4) Shake the test tube again and wait for a further 5 minute to make sure all possible denaturation has taken place 5) Make sure the colorimeter has the right filter in and is set to zero 6) Take each test tube and put a sample of each into a curvet (making sure the clear ends are not dirtied) place the curvet in to the colorimeter and take a reading 7) Record a reading for each sample and put into a graph and table discarding any anomalous results. Safety Ensure that great care is taken when handling copper sulphate. Ensure safety glasses are worn at all times and a lab coat to protect clothing. ...read more.

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