• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

An experiment to show the lowest concentration of copper (II) sulphate solution needed to bring about full denaturation of egg albumen.

Extracts from this document...


An experiment to show the lowest concentration of copper (II) sulphate solution needed to bring about full denaturation of egg albumen Introduction Egg albumen is a complicated protein that is more commonly know as egg white. This protein becomes denatured when copper (II) sulphate is added at a certain concentration. This is because the cations from the copper ions react with the amino acids carboxylate anions which form peptide bonds. This in turn affects the secondary structure of the egg albumen since there is a disruption in the peptide bonds and the protein reforms as a fibrous protein precipitating out of the solution and becoming opaque, the copper ions also react with the hydrogen in the amine group causing the primary structure to fail also. ...read more.


It can also be used to clean other apparatus. Egg albumen this is needed to perform the experiment 0.1 mol dm-3 copper (II) Sulphate solution I will use this to make all my concentrations, e.g. 0.05, 0.025 etc Test tubes Test tube rack Pipettes and syringes these will be used to measure certain amounts accurately Preliminary work From looking at the results I have gathered. It showed me the importance of the range of concentrations used. My results were reasonable I know this because they were similar 2 most of my class mates This also helped me narrow down the range of result I would take I decided that I would only take between0.005 and 0.00625 Prediction I predict that it ...read more.


3) Now add 5 cm3 of egg albumen to each test tube shake to mix and then wait for 5 minutes 4) Shake the test tube again and wait for a further 5 minute to make sure all possible denaturation has taken place 5) Make sure the colorimeter has the right filter in and is set to zero 6) Take each test tube and put a sample of each into a curvet (making sure the clear ends are not dirtied) place the curvet in to the colorimeter and take a reading 7) Record a reading for each sample and put into a graph and table discarding any anomalous results. Safety Ensure that great care is taken when handling copper sulphate. Ensure safety glasses are worn at all times and a lab coat to protect clothing. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Aqueous Chemistry section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Aqueous Chemistry essays

  1. Marked by a teacher

    Biology Practical Investigation to find the lowest concentration of Copper Sulphate solution that will ...

    5 star(s)

    This method was repeated for the other concentrations. Five petri dishes were set up and 5ml of egg albumen were placed in each. Three drops of 0.1M copper sulphate solution was placed into one of the petri dishes containing egg albumen. This method was repeated for the rest of the concentrations.

  2. Investigate the lowest concentration of copper (II) sulphate that will bring about the full ...

    This hypothesis is the reason for my prediction. Preliminary Tests Preliminary tests showed that five minutes was enough to allow time for denaturation to occur in the stronger solutions. The opaqueness of all the variables that had fully denatured were almost identical providing an easy comparison with the solutions that did not denature.

  1. Investigating the Effects of Increasing Copper Sulphate Solution Concentrations on the Germination of Cress ...

    I will then mix it to create a solution of 0.6mg/l This gives me a 0.6mg/l solution which I will use as one of my 7 investigation solutions. I use a micropipette because it gives me an accurate measurement for each solution, so there will be no mistakes in the strength of each solution.

  2. Denaturation of Egg Albumen.

    sulphate solution. Quantity - of every solution will be the same. Temperature - will be done at room temperature. Time - I will allow the same amount of time for each concentration to denature. Nothing extra added - nothing other than the solutions sated will be used.

  1. How much Iron (II) in 100 grams of Spinach Oleracea?

    Using a clean dropping pipette add distilled water (l) to the Volumetric flask until the bottom of the meniscus is touching the graduation mark. 10) Stopper the flask and invert it several times. 11) Full a 50 cm3 burette with 0.01 mol dm-3 Potassium Manganate (VII) (aq). 12) Using a 10 cm3 pipette transfer 10 cm3 of the spinach extract solution to a conical flask.

  2. Egg albumen experiment.

    sulphate solution. The concentrations required are; 0.10m, 0.09m, 0.08m, 0.07m, 0.06m, 0.05m, 0.04m, 0.03m, 0.02m, 0.01m, 0.00 molar. Refer to serial dilution table below: fig 1: serial dilution table Concentration required Mols Vol. Copper (II) sulphate Cm3 Vol. Distilled Water Cm3 Total volume Cm3 0.00 0.00 10.00 10.00 0.01 1.00

  1. What concentration of copper (II) sulphate brings full denaturation of egg albumen.

    1.5625*10�� 7.8125*10� 3.90625*10� 1.953125*10� > Each test tube has half the concentration of the one before it, which I achieved by using this method. 4. Take each of the 10 solutions, and add to the ten curvettes and mix with the egg albumen.

  2. Investigation to determine the lowest concentration of copper (II) sulphate solution that brings full ...

    I made this decision by using different amounts in my preliminary testing and found that this was a suitable amount to denature in this experiment in relation to the amount of copper (II) sulphate that I will use. * Concentration of the copper (II)

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work