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An experiment to work out the concentration of plant cell sap using potato tuber cells.

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Introduction

OSMOSIS PRACTICAL An experiment to work out the concentration of plant cell sap using potato tuber cells First of all and the most important are the safety precautions we will take throughout this experiment * Wear safety glasses to protect eyes * Handling the acids carefully * Careful while using kitchen knife (And all the simple things such as not running etc.) Another important aspect before we start is making the experiment a fair test. This includes making sure the volume of each acid is the same using a measuring cylinder. The same temperature for each individual solution will be the same as they will be done one after the other and left for the same amount of time in the same place. A significant temperature change in between each one is unlikely to occur, especially indoors. Also the 6 potato chips will be the same size (using a ruler). ...read more.

Middle

this was done using a cork borer. 2. We dried each piece and weighed them separately recording each weight in a results table. 3. We then placed each chip in the different concentrations 4. Next we labelled each glass tube with our initials and the correct molarity (eg.0.1M) 5. We left them to settle for a day 6. Then we dried them and weighed them once again 7. Finally we recorded the final weight in the results table and worked out the change in weight and the percentage change. 8. We did this experiment twice to get more reliable and better results that will in affect improve my conclusion. Conclusion Both of the graphs have a negative correlation, which we would expect. My prediction was that the molarity of the potato would be 0.3M. If we look at where the line of best fit passes at the point zero, we can see that it is approximately 2.5M, which slightly disagrees with my prediction although it is fairly close. ...read more.

Conclusion

There are a few things that I could have changed - I think we could have had a better method for drying the chips because if a chip isn't dried properly then there is much more water on the chip at the start than another chip. This may affect the results and therefore may end up affecting the conclusion. Another thing we could have improved was the to try and do each individual solution at the very same time. The problem with this is that we simply didn't have enough people to do this. We could have even looked at different variables such as trying different temperatures or stirring the solution, this would increase the rate and would be quicker, give us a better, wider range of results. The temperature at the time we did each set of experiments may have been different and may have affected the results. This also was a problem, keeping the temperature constant. Nevertheless, although there was the odd anomaly the pattern on the graphs was clear and stood out, therefore I think that they were still a good set of results. ...read more.

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