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An investigation into the effect of substrate concentration on the release of oxygen from hydrogen peroxide by catalyse in potato tissue.

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Introduction

An investigation into the effect of substrate concentration on the release of oxygen from hydrogen peroxide by catalyse in potato tissue. Introduction. A catalysts is a substance which speeds up a chemical process. Enzymes work best at optimum conditions, temperature and pH for example. This is because they are made out of protein. A certain enzyme is pacific to a certain reaction and cannot be used in any other reaction as it's shape and design is purely for one reaction. In a reaction the enzyme joins with the substrate, (the substance acted upon) to produce the complex. The enzyme has a fixed shape which connects with a certain substrate, this method is often referred to as lock and key as the enzyme and substrate fit together perfectly, much like a lock and key. The reaction depends on the fact that they fit together and if the enzyme is slightly disfigured or denatured then no change will be produced. This means that only a certain type of enzyme can fit a certain type of substrate. Once the bond has been made a reaction can take place. ...read more.

Middle

These repeats will ensure reliable results so that if one set of results should be unusual I will be able to take an average of the results. After this I will do the same thing with the rest of the percentages, making sure that everything else is constant, for example the temperature, by doing all the experiments at once so there cannot be a large change in temperature, the amount of catalyse by using the same amount of potato each time and the same surface area of the catalyse. This will ensure that the experiment is only testing one variable which is the concentration of substrate, hydrogen peroxide. Method. The plan was followed as closely as possible but the experiment went slower than anticipated and therefore I did not prepare as much as I would of liked I did not have enough time to prepare all the potato discs before the experiment began so only half the potato had been prepared and the rest was prepared as it was needed. Despite this, there was enough time to prepare all the hydrogen peroxide solution and this was put into separate beaker for each part of the experiment. ...read more.

Conclusion

Evaluation. Although this experiment did contain a few anomalies, which are clearly shown in the results table, this experiment, on the whole was generally accurate. The results obviously show the effect of substrate concentration on the release of oxygen from hydrogen peroxide by catalyse in potato tissue, and this, I believe, makes it a very productive experiment. If I were to do his experiment again I would try to make it more accurate by ensuring that the time intervals between taking down results during the experiment were taken precisely at the right time. And perhaps I would take these results at shorter intervals. I believe that this experiment used a competent method and therefore should not be changed but to better the experiment I would cut out as much experimental error as possible. To further this experiment I would investigate the concentration of substrate and the effect on the release of oxygen from hydrogen peroxide by different types of catalyse from different things. I would also alter the variable to investigate other things that effect the amount of oxygen produced, such as temperature conditions, pH, and mass or surface area of the potato. This will give me a better understanding of the conditions that catalysts need, and how they work. by ellie brett 1 ...read more.

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