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An investigation into the effect of temperature on anaerobic respiration in yeast.

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Introduction

An investigation into the effect of temperature on anaerobic respiration in yeast Planning: Area P Preliminary report. From my preliminary work I found out that I should equilibrate my yeast -glucose solution so that the results will be fair. I found out that the best way to do this was to place a boiling tube, filled with the suspension up to half way, into the water bath at the desired temperature. I will leave this for 5 minutes to let the suspension adjust to the temperature that it will be tested at. Thus, the test will be as fair as possible. I also discovered that to make the concentration of the yeast suspension, after dilution, accurate I needed to stir the suspension for 1 minute to thoroughly mix the suspension. Also, I found that I should mark the fermentation tube at 0.5cm intervals so that I could easily read off the amount of carbon dioxide produced over the time that the yeast is being experimented on. ...read more.

Middle

Also for the control I will put it in with the other test tubes so that it also receives the same conditions that the other results will receive. When the tin can has been heated it is inevitable that that the temperature of the can will drop and thus effect the experiment results. This is hard to combat without overheating the water and thus effecting the results too much. If the drop is very extreme (at higher temperatures) then I will try to heat the water back up to the required temperature using the Bunsen burner. Factors to keep constant: When I experiment I need to use the same concentration of yeast-glucose solution. This is so that the yeast does not produce too much or too little carbon dioxide compared with all the other results. So to make the experiment fair I must experiment with the same concentration of yeast-glucose solution in each different experiment. I must also allow the same amount of time for equilibration each time I start a new experiment. ...read more.

Conclusion

This affects the results, as the time taken for the experiment to be completed will be longer. Also the first part of the reaction in the yeast will be aerobic, as there is a small bubble that appears in the top of the fermentation tube when it is inverted. However, after the oxygen has been used up only anaerobic respiration takes place. There is very minimal change in the results due to this. Proposed results table and graph. I will than use this results table for every experiment but change the temperature in the top row to the desired temperature. Temperature: 15�C Time that the experiment takes to complete. Experiment 1. Amount Carbon dioxide produced- cm� Repeat. Amount Carbon dioxide produced- cm� Average 0.0 2.0 4.0 6.0 etc For the control I will leave it running until the actual experiment has finished, thus, it will get the same time for equilibration and experimentation. Results for the control. Temperature Time allowed for the experiment. Amount of carbon dioxide produced. cm� 15�C 37�C 45�C 60�C ...read more.

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