An Investigation into the Effect of Temperature on the Rate of Respiration in Yeast.

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An Investigation into the Effect of Temperature on the Rate of Respiration in Yeast.

Aim: My aim is to try to find out the different variety of temperatures affecting the rate of respiration in my coursework.

Introduction

My coursework is about the rate of the yeast respiration system, yeast is a living organism also known as fungus, which use enzymes and will increase the activity if the temperature was risen by. The enzymes will start to denature at 50?c or higher and it will be useless. It has been written and scientists have proved beyond result that enzymes work best at 37?; this is also the human body temperature.

Aerobic respiration is the release of energy from glucose or another organic substrate in the presence of Oxygen. Strictly speaking aerobic means in the air, but it is the Oxygen in the air, which is necessary for aerobic respiration. Also aerobic respiration releases much more energy from a given amount of food. The equation for the aerobic respiration

Glucose + Oxygen = Carbon Dioxide + Water + Energy

Aerobic respiration is when we measure we measure the rate of respiration by measuring the carbon dioxide given out. This process takes place in the presence of oxygen.

Prediction

I predict that when the temperature rises we will produce more carbon dioxide. I have to remember this will only happen till a certain point until the enzymes start to denature this is my prediction.

Hypothesis

Preliminary

Results

Temperature

No of bubbles after

No of bubbles after a minute

2 minutes

20

0

0

30

25

2.5

40

30

5

50

40

20

60

9

9.5

My preliminary results have proved that after 50ºC that the amount of carbon dioxide that was produced was cut down.
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This shows that enzymes in the yeast work in a particular way, the enzymes seem to slowly work in the 'Lock & Key' mechanism technique and it was used where enzymes fit in to substrate molecules. If the glucose and yeast both were heated a bit too much then they will vibrate and fall apart and then there will be not much carbon dioxide left to be produced.

Apparatus

Below are the apparatus I have been using throughout the experiment of the yeast experiment.

The experiments I have used are: Conical flask, yeast, thermometer, test ...

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