• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month
Page
  1. 1
    1
  2. 2
    2
  3. 3
    3
  4. 4
    4
  5. 5
    5
  6. 6
    6
  7. 7
    7
  8. 8
    8
  9. 9
    9
  10. 10
    10
  11. 11
    11
  12. 12
    12
  13. 13
    13
  14. 14
    14

An Investigation into the effect of Temperature on the release of Betalain from Beetroot Tissue.

Extracts from this document...

Introduction

An Investigation into the effect of Temperature on the release of Betalain from Beetroot Tissue By Oliver Jewell Problem The aim of this investigation is to see what if any affect temperature has on the release of Betalain from beetroot tissue. To carry out this investigation I am going to need the following equipment and materials. Apparatus Electric water bath - This will be needed to keep the water temperature consistent throughout the experiment at the various required temperatures. Thermometer - This will be used to check that the water bath is heating accurately at the required temperatures throughout the investigation. Colorimeter - This is what will measure the affect that the heat has on the membrane by measuring how much light passes through the solution. These are the apparatus that will be used to heat and record the data but in order to use these other apparatus must be used too; Test tubes Syringe (to accurately measure the fluid amounts) Cork borer (to shape the beetroot equally) Curettes Measuring cylinder Scalpel Materials Beetroot Distilled Water Method Cut out three pieces of beetroot about 2cms long using a cork borer. Place the cylinders of beetroot on a tile or board and using the scapulae cut into discs 5mm thick. Label 3 test tubes, A B & C for each of the temperatures to be tested. The temperatures required are 20, 30, 40, 50, 60, 70 and 80�c Put 10cm� of distilled water in each test tube Place the three test tubes for the required temperature in the water bath and heat to the required temp if needed Check the required temp has been reached using the thermometer to measure both the water bath and the test tubes temps Place the three pieces of beetroot in the three test tubes and leave for two minuets After the time is over remove the test tubes from the water bath and using the syringe which should be clean, extract 5cl from each solution to fill up ...read more.

Middle

After the averages have been recorded graphs can be drawn up and then analysed for correlation or anomalous results. Scientific theories can then be used to explain the results and then conclude the investigation. Changes in Method There were some problems whilst collecting the results which may have an affect on the findings from them. Firstly was with the temperatures of the water baths which we heated the beetroot in; these were less reliable than I had hopped as far as keeping the water at a consistent temperature. They could not hold the water at exactly 35�C, 45�C, 55� or 65�C etc so temperatures were recorded from around the right temperature, and that temperature recorded with them. I would also have liked the water baths to be as consistent as possible but I'm not sure they were as once they reached the required temperature they turned off. This may mean that the temperatures varied slightly over the five minuet period the beetroot was left to diffuse, however I still feel that the temperatures recorded are varied enough and close enough to the original aims to still be used to analyse and solve the problem. The Colorimeter's readings may also have an affect on the results. This is because they did not always read consistently. This could be because of smudges on either on the curettes or on the lens or perhaps due to the particles moving around in the solution. I feel, however that the data collected has been accurate enough and varied enough to analyse and solve the problem fairly. I also found that two minuets did not prove a sufficient amount of time for the diffusion to take place, and so I extended the time to 10 minuets. This is because I found that I had underestimated the rate of diffusion from the beetroot and that after just 2 minuets not very much dye had diffused at all and comparisons would be small. ...read more.

Conclusion

There would then be no heat or buffer to keep the temperature constant. Whilst this is a much more effective and accurate way of reaching the temperatures and conducting the experiment than using Bunsen burners or any of the other equipment the school could have provided, it was a bit disappointing that it couldn't hold its temperature. The poor precision of the water bathes could have had an effect on the data recorded. The experiments were supposed to be conducted at 25, 35, 45, 55 and 65�C but the real temperatures were from around these temperatures. This could have led to variation in the in the in the data collected as some of the error bars were quite large, for instance the changing temperatures could mean that once you returned to repeat the experiment the water bath would be at a different temperature to when you first recorded the results. Another factor affecting the difference in results could the position in the water bathe, if two different thermometers (the water bathes thermostat and the separate thermometer) are reading different temperatures then maybe the temperature isn't consistent throughout the water bathe at the same time. If one test tube was placed directly above the heater and another away from it they would have different temperatures leading to a deviance in the results. This lack of reliability may have had an effect on the conclusions as well as the results. On the first graph the error bars were clearly to large and needed editing to remove the anomalies and redo a more consistent line. The figures used for the graphs were suppose to be for the rate of reaction and to work this out the diffusion should have been constant, but if the temperatures weren't constant then its probable that the diffusion wasn't either. This could not be helped though and differences - although there were some anomalies were fairly consistent and showed enough reliability to be analysed, concluded and explained using Biological Knowledge. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Green Plants as Organisms section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Green Plants as Organisms essays

  1. Marked by a teacher

    How does temperature affect the permeability of a cell membrane in a beetroot.

    4 star(s)

    As the temperature increased more anthocyanin could be released in the 10 minutes allowed to release the pigment into the water. A copy of the graph using my results is below. As you can see from this graph my results give a definite negative correlation this means that the percentage of transmission becomes lower as the temperature increases.

  2. Marked by a teacher

    AN INVESTIGATION OF THE DIFFERENT SPECIES OF MAYFLY WITHIN THE POOL & RIFFLE

    4 star(s)

    mayflies in the pools than in the riffles I also noticed that there were no Ephemeridae in the pools or riffles. The amount of mayfly nymphs found in my real investigation were low compared to the amount of mayflies found in my preliminary The factors affecting the number of species

  1. Peer reviewed

    Effect of Temperature on Beetroot Membrane Proteins

    4 star(s)

    This would help to either confirm or possibly disprove the theory that the percentage transmission decreases as the temperature increases. It is possible that diffusion of red pigment across the partially permeable membrane will stop at a higher temperature - perhaps if there came to be a higher concentration of

  2. Photosynthesis Investigation

    - Set up the apparatus - Boil some water in the large beaker at approximate of 150 ml - When it starts to boil put the leaf inside the water (we put the leaf inside the water of boiling water to remove all the cuticles of the leaf)

  1. The factors affecting the rate of permeability in a cell membrane?

    of the experiment and so the temperature must be controlled to not go higher or lower then the desired temperatures. This will make it a fair experiment and the results will be more accurate By using a water bath instead of a Bunsen burner as this will keep the temperature

  2. Anexperiment to investigate the effect of temperature on the leakage ofAnthocyanin from beetroot tissue

    * Measuring cylinder- I will use this to measure out 10cc water each time. * Digital scales- to check the mass of the beetroot piece. I will now outline my method for my experiment:- 1. I will put the test tubes with 10cc of water (which I measured with a measuring cylinder)

  1. A Plan of an Investigation of the Effect of Temperature on the Volume of ...

    However, if some have been left in for a shorter period of time than others, the pigment within them will have had less time in which to leak out and so the percentage of light that will be able

  2. Broad Bean Investigation.

    as there will no really be any big major development over 2 days, I will be able to record at least all of what happens to the broad bean during the length of the investigation. 5. For the risk of tampering this I cannot control but precautions taken to ensure

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work