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An Investigation to find out the affect of increasing amount of catalase on hydrogen peroxide

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Introduction

An Investigation to find out the affect Of increasing amount of catalase on hydrogen peroxide General Background In all cells an enzyme called catalase can be found, this enzyme breaks down various substrates to produce products. This investigation will show the affects of catalase on the substrate hydrogen peroxide. The equation below shows the reaction that will take place during the experiment, Catalase 2H2O2 2H2O + O2 Throughout this investigation there will be a record kept of how much gas is produced for the reaction, from this we can work out the rate of reaction that occurs when this enzyme is at work. Apparatus List * Clamp and Stand * Conical Flask (100cm�) * Gas Syringe (100cm�) * Beaker (100cm�) * Beaker (200cm�) * Syringe (10cm�) * Stop Watch * Glass Tile * Ruler * Thermometer * Knife * Glass Rod * Cork Borer (x7) * Potato * Hydrogen Peroxide * Safety Goggles * Lab Coat * Surgical Gloves (if required) Hypothesis The prediction is that when the enzyme concentration is increased the rate of reaction between the catalase and hydrogen peroxide will also increase. It is thought that as you double the concentration you will also double the rate of reaction. To see if the prediction is correct the amount of gas collected throughout the experiment at set intervals of time will be used when the reaction takes place. Pilot Study Before carrying out the real experiment a pilot study was carried out to work out what measurements would be the best to use. ...read more.

Middle

1 5 10 15 20 24 29 32 36 39 42 2 3 7 10 14 17 20 22 25 27 30 3 5 8 13 17 20 23 27 30 32 34 Av 4.3 8.3 12.6 17 20.3 34 37 30.3 32.6 35.3 Amount of gas given off with 35 disks (cm�) 1 5 8 13 17 22 26 30 34 37 40 2 5 9 15 19 24 27 31 35 39 42 3 6 8 14 17 23 26 32 34 38 41 Av 5.3 8.3 14 17.6 23 26.3 31 34.3 38 41 Results Tables for use in analysis From graph 3 on a previous page the table below could be completed. Volume of Gas (cm�) N� of disks After 30 sec After 20 sec 25 - 30 sec Per min 15 0.562 0.375 0.187 2.244 20 1.312 1.062 0.25 3 25 2.5 2.187 0.312 3.744 30 4.25 3.562 0.688 8.256 35 5.25 4.5 0.75 9 Now because all the data for the above table has been filled in the table below could also be filled in. N� disk Vol of gas per min (cm�) Expected Vol compared with 35 disks Difference % Difference 15 2.244 15/35 x 9 = 3.86 -1.616 -41.87% 20 3 20/35 x 9 = 5.14 -2.14 -41.63% 25 3.744 25/35 x 9 = 6.43 -2.686 -41.77% 30 8.256 30/35 x 9 = 7.71 +0.485 +6.76% 35 9 Analysis By looking at all the graphs, which have been created from the set of results on a previous page I am are able to come up with various conclusions. ...read more.

Conclusion

* From the view of problems with the potato there are various, 1. Due to different potatoes having to be used throughout the experiment there could have been different concentrations of catalase found in each. So therefore if there were more catalase in one potato this would have caused the reaction to take place faster. 2. Some of the cylinders of potato that were being used had area's which had begun to go off so this could have cause a 'deformation' throughout all the potato and it may have affected the catalase in the potato. Due to this there may have been areas where the catalase either didn't react fully or didn't react at all. As well as the limitations with the actual experiment there are limitations with recording the data, * If the line on the gas syringe was in the middle of two markers some people would read it off as .5 where as other people would round it either up or down so that could alter the graphs and therefore alter the rates of reaction. * The readings being taken every 30seconds was unable to be accurate because as the stopwatch hits the 30seconds the gas syringe could move by the time the reading is being taken. This can't really be made more accurate unless two people took the reading with one person watching the stopwatch and the other taking the reading. Lyanne Craft AS Biology Coursework 1 Lyanne Craft AS Biology Coursework Lyanne Craft AS Biology Coursework Lyanne Craft AS Biology Coursework Lyanne Craft AS Biology Coursework ...read more.

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