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Anaerobic Reaction of yeast

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Introduction

P O A E SPaG TOTAL Assessment Title Anaerobic Reaction of yeast_ Date _06/17/01 Candidate's name_______MichaelAkers______________________________________________ Candidate's Number _____________________________________ Centre Number 58615 I am going to investigate the anaerobic reaction of Yeast. Anaerobic respiration requires: Glucose ==> Co2+Alcohol. Whilst Aerobic respiration, which we use to breathe, requires: Glucose + Oxygen ==> Co2+H20+ heat energy. To observe and record results of this reaction I will measure the amount of Co2 this experiment produces. There are a number of variables I need to consider beforehand that could dramatically affect my experiment. I have also made predictions where appropriate Temperature This can make the rate of reaction speed up or slow down. It will noticeably slow down the reaction when the water is cooled. But the Yeast will speed up until a point where it cannot survive anymore. I would expect my graph to show the Yeast speeding up and then dying. As I heat the water, the particles start to move faster, this makes them collide at a faster speed, speeding up the rate of reaction. Amount of Yeast I will keep the amount at a constant by using a test-tube to measure out an exact amount. ...read more.

Middle

Put a bung and pipe in the end ? fill the beaker with water to the right temperature ? Fill graduated tube water ? Put the tube into the test-tube ? Put the boiling tube in the beaker ? Leave for 3mins to get to temperature ? Time for 5mins ? Measure amount of Co2 produced. ?Then I will alter to a different temperature in this case my temperature is altering 10 each time so I will use the kettle to alter this temperature making sure it is the right temperature by using a thermometer. ?I will use the same amount of yeast for each 'run' to eliminate solution variation. As I am obtaining these results, I will make sure I use the correct amount of ingredients and make sure I keep my variables (i.e. amount of yeast the same throughout experiment) to what I have predicted they should stay at in experiment. I will record my results in a table as underneath, but I may need to repeat some results, if they look out of place when compared to my results or conclusion. Temp Time Run1 (cm) Run2 (cm) Average (cm) 20 5mins 30 5mins 40 5mins 50 5mins 60 5mins 70 5mins 80 5mins Obtaining evidence After I conducting my experiment to record my ...read more.

Conclusion

I think that I can form the conclusion that 'the yeast can only perform anaerobic respiration past a certain temperature (20) and under a certain temperature (80)'. As temperature was my chosen variable to alter and measure this is what I have discovered. I think if I could extend my study of this I could make changes to keep in my experiment set up to keep nearly all my variables at a constant, e.g. I could use a more reliable source of heating the water other than a kettle, this would greatly improve the reliability of the experiment, I may get more accurate and even better results than I have now by taking recordings of the Co2 in the yeast every 5 degrees, this would give me a better indication of whether the results are on course to being accurate and I could have a better chance at pin pointing when Co2 stops being produced. I think I experiment was a success I have spotted when the yeast stops anaerobically reacting and when it starts anaerobically reacting. I have found out that the enzymes in the yeast start to rapidly speed up when they are heated but too much heat will kill them as we have seen in my experiment. ****************************** Page 1 ...read more.

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