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Analyse how temperature affects enzyme activity.

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Introduction

Effect of enzyme activity and temperature. Elaine Raven Statement: I am going to analyse how temperature affects enzyme activity. Method: I will set up the apparatus as show in the diagram, making sure they are all clean of foreign substances. I will then cut the potato. Once I have put on my safety goggles I will measure out 50mls of hydrogen peroxide, then heat it using the flask, thermometer, Bunsen and tripod to one of our five test temperatures. Then out the hydrogen peroxide into the chronicle flask and secure the bun as soon as possible. Then after five minutes I will measure how much the water in the measuring cylinder has decreased. ...read more.

Middle

I will use a new batch of 50mlsof hydrogen peroxide each time. I will make sure that the pH conditions are the same each time (pH 7). In this experiment I am choosing to explore one of the factors that effect enzyme activity. I am aware that there are also other factors, which I will have to monitor to make sure the experiment is fair. Preliminary: I know that factors such as temperature, pH levels, and substrate concentration effect enzyme activity that work at 40oC. So when washing clothes with biological washing powder that contains enzymes, you must wash it at the temperature they instruct other wise the enzymes will denature and not work. ...read more.

Conclusion

The graph shows that the enzyme potato catalyse works best (produces the most oxygen at 30oC. All the results are coherent so I can rule out the possibility of any anonymous results. Evaluation: I feel my experiment was a well controlled, as there were no anonymous results. I feel the experiment was a success. If I could do the experiment again I would use a gas syringe; and do it on a bigger scale leaving leaning the enzymes longer to react leaving me with a full bell graph. This would've told me when the enzymes denatured. It was a slightly inaccurate experiment due to it being hard to cut the potato into equal sizes. I would use a gas syringe because it would have measured the amount of oxygen given off accurately. ...read more.

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