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Are all Saccharides Reducing Sugars

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Introduction

Are all Saccharides Reducing Sugars? I am going to do an experiment to find out if all saccharides are reducing sugars Equipment Test tube's Galactose Sodium Bicarbonate Maltose Benedict's Solution Sucrose Water Bath Lactose Test Tube Rack Glucose Hydrochloric acid Method I will first set up the experiment by placing 5 test tube's in the test tube rack, in each separate test tube I will put a different saccharide and this will be measured to 2cm3 for each. I will then one by one add a dropper full of benedict's solution to each test tube and then place the test tube into the water bath for 2 minutes; I will repeat this process for each sugar. ...read more.

Middle

test tube out of the water bath and check to see if there has been a colour change, we then record the results. Prediction My prediction is that at least one or more of the saccharides will be non-reducing sugars. I am predicting this because 2 of the sugars are disaccharides this means that they are made up of two sugar molecules, I think that because of this the sugar molecules will be bonded to each other and thus unable to react with the benedict's solution. Results Saccharides Change? Colour? Change after the hydrochloric acid and sodium bicarbonate Glucose Yes Orange Not Needed Maltose Yes Orange Not Needed Sucrose No None Changed to Orange Lactose Yes Orange Not Needed Galactose Yes Orange Not Needed Analysis ...read more.

Conclusion

Benedict's solution is made up of copper sulphate and has a positive charge of Cu2+ this means that when the Benedict's solution is added to the sugar if it is a reducing sugar the electrons from the oxygen are released to the copper (II) ions, this is because oxygen is very electronegative and causes electrons to be unevenly distributed in the carbohydrate. They gain electrons from the molecule and copper (I) ions, Cu+ are formed. Copper (I) oxide is insoluble and so it produces a red precipitate Cu2++e- Cu+ Evaluation Overall I think my experiment was pretty fair and I got the results that I expected as I predicted. Craig Thompson 12R ...read more.

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