• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Aseptic Technique – Growing Microorganisms

Extracts from this document...

Introduction

Aseptic Technique - Growing Microorganisms Aim: The aim for this topic is to grow microorganisms using the Aseptic technique. Equipment: Agar plate Wire loop Heatproof mat Tissue Safety goggles Bunsen burner Disinfectant Beaker Lactobacillus casei (bacteria) Masking tape Matches Ethanol Marker Prediction: I predict for this experiment that the most of the colonies of the bacteria would grow on the streak 1 because on the first number of scrapes onto the agar dish would give thousands and thousands of Lactobacillus casei bacteria, for the second number of scrapes would have probably only hundreds of bacteria. A disadvantage for having this many in the first number of scrapes is the amount of nutrition the bacteria would get from the agar so many of the bacteria would die out. There are still many bacteria left so the chances are that the first stroke would get the most colonies in the agar dish. Risk Assessment: Biological: The loop must be kept away from the face because is contains the bacteria that's used in the experiment. ...read more.

Middle

5) The bacteria around the neck of bottle must also be destroyed, to do this I had to run the bottle around the flame. All the bacteria would die because of the heat from the flame. 6) Scrape the bacteria inside the bottle using the wire loop then put the bottle again around the flame then put it on the table. 7) My partner then took the wire loop so I can put the lid back on the bottle. I had then opened the agar dish about half way or about 45degrees. 8) My partner then scraped the bacteria on the agar dish in sections of lines as shown below in the diagram: Agar Dish Streak number 9) I had closed it and used to masking tape to keep it in place. My partner had then put the wire loop into the disinfectant beaker When I had finished taking the bacteria out of the bottle and putting it onto the agar- dish I labelled our work with (D-M) written on it so we can tell out which is our work from the class. ...read more.

Conclusion

Another problem that I had encountered was the burning the wire loop; it took a long time before the wire loop became red-hot, the reason for this problem is because the Bunsen burner had its hole blocked with paper but we had not noticed so this means it does not have enough oxygen to create a roaring flame. Strengths: 1) I have achieved my aim. 2) My prediction was correct. 3) I had worked well with my partner and had completed my work successfully 4) I had used the correct method. 5) I had finished the experiment with 10 minuets to spare. Weakness: 1) I had not managed to grow as much bacteria colonies as I had hoped. 2) I had not used enough disinfectant to clean my table leaving parts of the table dry, this make the experiment not 100% Aseptic. 3) I had not used much safety methods for instance wearing gloves when handling the disinfectant. 4) The wire loop took quite long to become hot red. 5) I did not scrape many bacteria out of the bottle as I was in a rush. Improvements: 1) Try to grow more colonies of bacteria. 2) ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Living Things in their Environment section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Living Things in their Environment essays

  1. Marked by a teacher

    Research question - Is using dogs for work ethical?

    5 star(s)

    1.Yes- they are honest 2.Yes 3.Yes 4.Yes 5.Yes Examples of dogs having human characteristics & behaviours: 1. A dog once died a few weeks after its owner died 2. Paris Hilton dresses up her Dog to look like her- and they have a conscience 3.

  2. Investigating the effect of four antibiotic agents on gram positive and gram negative bacteria.

    * Always work near a Bunsen burner, as it is more convenient to sterilise instruments. It also creates an updraft of air, which helps to prevent microorganisms from the worker being transferred to the culture. * Sterilise all implements before use.

  1. Aseptic Technique.

    I then removed the cap. 4) With the cooled loop, I removed a small amount of agar jelly. 5) I then flamed the neck and recapped the bottle. 6) I picked up the petri dish and spread the agar jelly that was on the loop to the base of the petri dish.

  2. The main aim in the life science lessons is to learn how to handle ...

    Procedures (cont'd) Precautions-Before doing anything, remember to put on lab coat and gloves and surface sterilize. Always open the petri dish at about 45 degrees, never talk while doing this and close the dish immediately after that to prevent contaminations. Preparation of LB agar plate-Firstly, we divided the petri dish into 3 main section, a half and two quarters.

  1. Introduction to bacteria

    Antibiotics are used to treat disease caused by bacteria or to prevent these diseases from occurring in the first place. The following list gives important antibiotics produced by bacteria. Penicillin is not on the list because it is produced by a fungus called Penicillum chrysogenum.

  2. Using the streak plate method, compare the effect of two different brands of toothpaste ...

    Draw around the area of inhibition and count how many squares the clear area covers. Do not invert the plate and repeat the procedure for each bacterium to be tested. Test each toothpaste twice. The inhibition zone area indicated the susceptibility or resistance of the bacteria to the toothpaste being tested.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work