• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Aseptic Technique – Growing Microorganisms

Extracts from this document...

Introduction

Aseptic Technique - Growing Microorganisms Aim: The aim for this topic is to grow microorganisms using the Aseptic technique. Equipment: Agar plate Wire loop Heatproof mat Tissue Safety goggles Bunsen burner Disinfectant Beaker Lactobacillus casei (bacteria) Masking tape Matches Ethanol Marker Prediction: I predict for this experiment that the most of the colonies of the bacteria would grow on the streak 1 because on the first number of scrapes onto the agar dish would give thousands and thousands of Lactobacillus casei bacteria, for the second number of scrapes would have probably only hundreds of bacteria. A disadvantage for having this many in the first number of scrapes is the amount of nutrition the bacteria would get from the agar so many of the bacteria would die out. There are still many bacteria left so the chances are that the first stroke would get the most colonies in the agar dish. Risk Assessment: Biological: The loop must be kept away from the face because is contains the bacteria that's used in the experiment. ...read more.

Middle

5) The bacteria around the neck of bottle must also be destroyed, to do this I had to run the bottle around the flame. All the bacteria would die because of the heat from the flame. 6) Scrape the bacteria inside the bottle using the wire loop then put the bottle again around the flame then put it on the table. 7) My partner then took the wire loop so I can put the lid back on the bottle. I had then opened the agar dish about half way or about 45degrees. 8) My partner then scraped the bacteria on the agar dish in sections of lines as shown below in the diagram: Agar Dish Streak number 9) I had closed it and used to masking tape to keep it in place. My partner had then put the wire loop into the disinfectant beaker When I had finished taking the bacteria out of the bottle and putting it onto the agar- dish I labelled our work with (D-M) written on it so we can tell out which is our work from the class. ...read more.

Conclusion

Another problem that I had encountered was the burning the wire loop; it took a long time before the wire loop became red-hot, the reason for this problem is because the Bunsen burner had its hole blocked with paper but we had not noticed so this means it does not have enough oxygen to create a roaring flame. Strengths: 1) I have achieved my aim. 2) My prediction was correct. 3) I had worked well with my partner and had completed my work successfully 4) I had used the correct method. 5) I had finished the experiment with 10 minuets to spare. Weakness: 1) I had not managed to grow as much bacteria colonies as I had hoped. 2) I had not used enough disinfectant to clean my table leaving parts of the table dry, this make the experiment not 100% Aseptic. 3) I had not used much safety methods for instance wearing gloves when handling the disinfectant. 4) The wire loop took quite long to become hot red. 5) I did not scrape many bacteria out of the bottle as I was in a rush. Improvements: 1) Try to grow more colonies of bacteria. 2) ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Living Things in their Environment section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Living Things in their Environment essays

  1. Marked by a teacher

    Research question - Is using dogs for work ethical?

    5 star(s)

    in Dogs, the characteristics & the ethics from the Human and the Dog point of view so that I had a variety of questions to make my experiment more interesting and have a variety of answers. Data Dog Questionnaire with results: Do you have a dog as a pet?

  2. Aseptic Technique.

    This is because one plate had not been contaminated and the other did which was positive as I did have a result. I found streaking the plate difficult because there were too many things to do at once such as holding the agar jelly with the lid off in one hand and flaming the loop in the other.

  1. Aseptic Technique.

    Label the Petri dish 5. Put 1cm3 of each sample into separate Petri dishes. Use the same pipette for each sample. Don't leave the lid off the Petri dish for too long as other bacteria may contaminate the water sample in the Petri dish.

  2. Investigating the effect of four antibiotic agents on gram positive and gram negative bacteria.

    * Sterilise all implements before use. Metal instruments should be heated to red heat before and after every contact with microorganisms. * Petri dish lids should not be laid on the bench, but held while the vessel is being used. * The necks of all tubes, bottles and flash should be passed through the Bunsen flame before and after all operations.

  1. The main aim in the life science lessons is to learn how to handle ...

    As for the bacteria which was collected from the thumb, there were many colonies and a few big confluent growths in the before section. There were a couple of colonies in the after section. Results (cont'd) Petri dish 3 Sample- Vitagen Observation There was only one very small colony in

  2. Introduction to bacteria

    Since they can reproduce, biological agents have the unique potential to make an environment more dangerous over time. If used for hostile purposes any disease-causing micro organism could be considered a weapon. For the purpose of warfare, specific characteristics of certain agents make them more likely to be used than others.

  1. Survey of Bacteria and Moulds around the school, and

    Common room 336 400 368 0 0 0 11 L8 292 308 300 2 3 2.5 12 Pool Deck 288 308 298 0 0 0 13 Oval 188 188 0 0 Table 2: standard error of means Average number of mould colonies Standard error of mean of mould colony counts

  2. Using the streak plate method, compare the effect of two different brands of toothpaste ...

    Remember to place the piece of agar that has been removed by the cork borer somewhere safe. Next, insert 1cm3 of one of the toothpastes into the hole you will have just made using a sterile syringe. Try and keep the toothpaste as neat as possible in the hole without it spilling over the sides.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work