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Aseptic Technique – Growing Microorganisms

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Introduction

Aseptic Technique - Growing Microorganisms Aim: The aim for this topic is to grow microorganisms using the Aseptic technique. Equipment: Agar plate Wire loop Heatproof mat Tissue Safety goggles Bunsen burner Disinfectant Beaker Lactobacillus casei (bacteria) Masking tape Matches Ethanol Marker Prediction: I predict for this experiment that the most of the colonies of the bacteria would grow on the streak 1 because on the first number of scrapes onto the agar dish would give thousands and thousands of Lactobacillus casei bacteria, for the second number of scrapes would have probably only hundreds of bacteria. A disadvantage for having this many in the first number of scrapes is the amount of nutrition the bacteria would get from the agar so many of the bacteria would die out. There are still many bacteria left so the chances are that the first stroke would get the most colonies in the agar dish. Risk Assessment: Biological: The loop must be kept away from the face because is contains the bacteria that's used in the experiment. ...read more.

Middle

5) The bacteria around the neck of bottle must also be destroyed, to do this I had to run the bottle around the flame. All the bacteria would die because of the heat from the flame. 6) Scrape the bacteria inside the bottle using the wire loop then put the bottle again around the flame then put it on the table. 7) My partner then took the wire loop so I can put the lid back on the bottle. I had then opened the agar dish about half way or about 45degrees. 8) My partner then scraped the bacteria on the agar dish in sections of lines as shown below in the diagram: Agar Dish Streak number 9) I had closed it and used to masking tape to keep it in place. My partner had then put the wire loop into the disinfectant beaker When I had finished taking the bacteria out of the bottle and putting it onto the agar- dish I labelled our work with (D-M) written on it so we can tell out which is our work from the class. ...read more.

Conclusion

Another problem that I had encountered was the burning the wire loop; it took a long time before the wire loop became red-hot, the reason for this problem is because the Bunsen burner had its hole blocked with paper but we had not noticed so this means it does not have enough oxygen to create a roaring flame. Strengths: 1) I have achieved my aim. 2) My prediction was correct. 3) I had worked well with my partner and had completed my work successfully 4) I had used the correct method. 5) I had finished the experiment with 10 minuets to spare. Weakness: 1) I had not managed to grow as much bacteria colonies as I had hoped. 2) I had not used enough disinfectant to clean my table leaving parts of the table dry, this make the experiment not 100% Aseptic. 3) I had not used much safety methods for instance wearing gloves when handling the disinfectant. 4) The wire loop took quite long to become hot red. 5) I did not scrape many bacteria out of the bottle as I was in a rush. Improvements: 1) Try to grow more colonies of bacteria. 2) ...read more.

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