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Aseptic technique

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Introduction

Aseptic technique

Introduction

The principle of aseptic technique is to prevent the introduction of unwanted micro-organisms into your culture or environment. When micro-organism are in the air, they can enter the culture when the lid is removed. The micro organism ill be culturing is yeast which is a fungi.Pure yeast cultures are grown in a medium of sugars, nitrogen sources, minerals, and water. The final product may take the form of dried yeast cells. Fungi are a group of cells or multi cellular cells which feed direct from absorption of nutrients.

Aim:

During the course of this project I aim to successfully grow a yeast culture and sterilise the equipment that I use. I also hope to gain the skill of aseptic technique and hope to use it in high level science.

Safety

There are many safety issues that arise when dealing with chemicals and laboratory’s that is why when conducting an experiment all safety aspects need to be thought about before carrying out the experiment otherwise someone could occur contamination or a serious injury when conducting the experiment. One of the main parts of the human body when carrying out chemical experiments are they eyes do to the fact they can easily be damaged and are clearly one of the most  delicate exposed parts of the human body. To protect these fragile biological sensors a form of protection is crucial.

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Middle

The inhaling of a micro biological culture could lead to serious internal injury such as infections that is why it crucial not to open the culture once it has grown and seal it tightly so it will not open without extreme force. In the case that a culture is exposed immediately evacuate the class room and contact a lab technician immediately.

Method

When the experiment was carried out the first thing that needed to be attended to was having all the correct equipment and materials at hand. The following equipment and materials were gathered:

  • A metal wire- a metal wire which has a high melting point created out a metal such as iron and is made into a wire with diameter of a about 2mm but yet is malleable and yet maintains high strength.
  • Bunsen burner- A laboratory gas burner invented in 1855 by Roberts Bunsen. The Bunsen burner has a vertical metal tube flows through. The vertical tube has a while located at the bottom of it which is used to admit air. When the whole is closed a yellow safety flame is displayed. Where as when the whole is open it displays a power dull blue flame with a faint blue outer flame with a vibrant blue core used u for combustion and heating.
  • A bottle of pure yeast- A tube shape bottle with a screw cap containing pure yeast.
  • Agar plate-A plate of agar that contains the correct nutrients for the yeast to grow.
  • Petra dish- Circular dish with a lid which is transparent.
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Conclusion

I think that the method and the equipment and the materials for this experiment are great for maximum accuracy but they do not eliminate the chance for human error.

The only problem with the method of this experiment is that it should be changed for two people instead of one. This is because the method clearly needs another person for example holding a wire I one hand and then unscrewing and flaming a bottle with the other hand is clearly endangering people that is why I personally think this experiment should be conducted between to people.

Over all out of the whole experiment I enjoyed the theory the most due to the fact that it enhanced me knowledge about preparing accurate chemical solutions and it showed me it just wasn’t a basic skill but a crucial skill which involved a high level of accuracy I also enjoyed conducting the experiment even though I encountered many errors I enjoyed finding a solution to them. I also think that this experiment has challenged me mentally in every academic way. It has also helped me to develop personal thinking strategies.

During this experiment I learnt quote a lot and enhanced my general knowledge of chemicals as a whole and I learnt practical skills of preparing chemical solutions which I will be able to use in the future.

Over all this experiment has been an interesting and an enlightening experience which I have gained many skills and much knowledge even though I had encountered many problems I enjoyed the experiment thoroughly and would gladly repeat it.

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