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At what temperature does Catalase work best at?

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At what temperature does Catalase work best at? Aim: To see what temperature Catalase works better at. Prediction: I predict that at a low temperature the Catalase will work slowly but the work rate will increase as the temperature does. I think that it will work best at 37Oc because that is the temperature of the human body. I also think that it will start to denature at 50Oc. Background information: Enzymes: * Are Complex proteins that cause a specific chemical change in other substances without being changed themselves. * Exist in saliva, gastric juices, pancreatic juices, intestinal juices and intestinal mucosa. * Change starches, proteins and sugars into substances the body can digest. * Allow many chemical reactions to occur within the homeostasis constraints of a living substance. * Function as organic catalysts. * Are formed in the cells of any living creatures, plants, bacteria and microscopic single celled organisms. * Are broken down normally so not a threat to anything. * Are categorized in accordance to the compounds they act on: Lipases-fat into glycerol. Catalase-Hydrogen peroxide into water and oxygen. Amylase-Starch into glucose. Protease-Proteins into amino acids. Plan: 1. Measure out 5g of potato. 2. Crush potato with pestle and mortar. 3. Set up Bunsen burner, gauze, tripod and heat proof mat. 4. Fill beaker with 200ml's of water and place on top of tripod. 5. ...read more.


* I made sure that when I was boiling the water I was standing next to it so I could warn people that the water was hot and to tell them to stay a safe distance away. * When using the scalpel to cut the potato I always made sure that people knew in case I slipped and hurt them. * I cut the potato slowly and carefully so I did not cut myself with the scalpel. * I made sure that if I came into contact with the Hydrogen Peroxide I washed my hands immediately so I did not burn my hands because of the concentration. Apparatus: For my experiment I had to use certain pieces of apparatus. These apparatus are: Bunsen Burner, Tripod, Heatproof mat, Gauze, Scalpel, Thermometer, 3 x Boiling tube, Beaker, Test tube holder, Tile, Conical flask, Bung, Scales, Syringe, Potato, Hydrogen Peroxide, Pestel, Mortar. Observations: I intend to observe and record in my experiment. I wish to observe and record how much gas is coming off the Potato and Hydrogen Peroxide at the different temperatures. The temperatures are: 10, 20, 30, 40, 50, 60 and 70Oc. I intend to record these three times at each temperature. Method: As plan. Results: A table to show how much oxygen was given off during each reaction. Temp (Oc) 10 20 30 40 50 60 70 Oxygen (cm3) ...read more.


During my experiment and as my results show I did have a few odd results. This may be due to many factors as I have pointed out in the above statements. When reading the syringe I did read it to the best of my ability but the syringes do not have a very good scale for pinpoint accuracy. They only measure every 1 cm3. When I was measuring the Hydrogen Peroxide I used a conical flask, which is not very accurate because it only goes up in 50 cm3. Even though I used 100 cm3 it was not a very accurate way of measuring the chemical. I did however measure the potato correctly and accurately by using electric scales. A clear conclusion can be made even though I have some results that are out of place. That is that between 0-40 degrees the rate of reaction is increasing, at 40 degrees it reaches its ultimate rate of reaction then it starts to denature and the rate of reaction falls until it reaches zero. I could extend my experiment by heating only the potato or the Hydrogen Peroxide so I can find out whether the temperature of the substance being broken down changes the rate of reaction. Also I would like to find out what has the most Catalase in out of liver, potato and bread like some of my other colleagues in my class. ...read more.

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