• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month
Page
  1. 1
    1
  2. 2
    2
  3. 3
    3
  4. 4
    4
  5. 5
    5
  6. 6
    6
  7. 7
    7
  8. 8
    8
  9. 9
    9
  10. 10
    10

Biology- enzyme coursework

Extracts from this document...

Introduction

Biology Coursework: Enzymes By Kerry Douglas Investigate the Effect of Temperature on the Action of the Enzyme Amylase This expirment was set up to find the effect of different tempetures on the enzyme: Amylase. We had two different variables: Independent and Dependent. Independent Variable: The factor to be changed in the investigation. In the this practical it was the temperature. Using water baths we had a range of six temperatures - 25�C to 50�C with 5�C intervals between each. Dependent Variable: The factor to be measured during the investigation. In this practical it was the time taken for starch to break down. We did this using a stop-clock and we were able to calculate the rate of reaction. Rate of reaction= 1/time taken (s-1) Controlled Variables: These are the factors which must be kept constant to create a fair test. In our investigation we used the: 1. Same enzymes 2. Same substrate 3. Same volume of enzyme and substrate 4. Same time intverals for measurment 5. Same volume of Iodine 6. Same concentration of Amylase Hypothesis : In this invesigation I predict that as the temperature increases, the rate of reaction will increase, but this will only occur up to a certain point and temperature or the enzyme will become denatured- causing the rate to decrease drastically. ...read more.

Middle

We chose these temperates because they fall on what we belive to be the optimum temperature. The procedure was repeated three times to ensure the results would be reliable and so we could identify any anomalous results. Rate of Reaction(s) X1000 Temperature �C Method Apparatus/Chemicals: * Six waterbaths * Beakers * Syringes (5 cm3) (5ml) * Spotting Tile * Stop Clock * Saftey goggles * Dropper (pipette) * 1% Starch suspension * 0.1% Amylase solution * Iodine Solution Procedure: 1. Switch on the water baths and allow them to reach the temperatures needed. 2. Place amylase in a beaker and starch in a different beaker into each water bath. The water baths will have different temperatures. 3. Using syringes to measure the volumes, place 5 cm3 starch suspension in one beaker and 5cm3 of Amylase in a separate beaker. Make sure you use different syringes so there is no coss-contamination of substances. 4. Place one drop of iodine in each depression of the spotting tiles using a pipette. Saftey goggles should be worn as iodine is dangerous to the eyes. 5. Pour the contents of one beaker i.e Pour the starch into the amylase, then swirl the liquid to ensure thorough mixing. ...read more.

Conclusion

Sources of Error This procedure was suitable because the iodine test produced results that were easily observed to determine the rate of reaction and results were as expected. The set of results in the replicas are similar but not identical, this is due to sources of error. For example: * Differences in drop size therefore more/less iodine mixture added will affect the colour. * Timimng. It was difficult to start the clock/timer and mix the solutuion at the same time * It is difficult to judge and point due to simiular colour in the last few spots, it was personal interpretations of the colour. Improvments * Use a syringe instead of a dropper to produce equal volume (0.1) ml. * Work in pairs to improve the timing- one person mixes, one person sets the clock. * Use spotting tiles of the same shade (all white or all cream) so the colours will be the same. * Or use a colourmeter machine to judge the colours. Anomalous Results An odd result was 24�C - 660�C. It was faster than the others due to the possiblity that there was too little starch or too much enzyme. At 44�C - 870�C is slower than the others, this may be because there is too much start or too little enzyme. Both of these are accurate for measurment error. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Living Things in their Environment section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Here's what a teacher thought of this essay

4 star(s)

A good piece of coursework which is well thought out and executed. A few minor issues to be tightened up on and made clearer. 4 stars.

Marked by teacher Louise Star 08/01/2013

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Living Things in their Environment essays

  1. Marked by a teacher

    A2 Biology Coursework -Investigation into the effect of different concentrations of antibiotics on the ...

    4 star(s)

    solutions Glass rod 1 To spread bacteria equally, to reduce any confounding variable. Ethanol solution 1 5cm3 To burn glass rod to kill any unwanted bacteria Incubator 1 To incubate bacterial solution at optimum temp Permanent marker 1 To label test tubes and Petri-dish Corks 11 To seal top of

  2. Marked by a teacher

    In this experiment, mung bean seedlings and Brine shrimp eggs were used to study ...

    4 star(s)

    Temperature has a profound effect on the whole organisms as it will affect the enzyme activity in the organisms and thus, the development of the organisms. Salinity is the saltiness or dissolved salt content of a body of water. It is a general term which is used to describe the

  1. Marked by a teacher

    An Investigation into a Woodlice's Preferred Choice of Environment.

    3 star(s)

    INTRODUCTION This experiment is to test the preferred environments of woodlice. As is said above, it is said that woodlice prefer fairly dark and damp environments to live in as compared to drier and lighter ones. By using a choice chamber combining these four different conditions, and by using cotton

  2. What Factors are responsible for the success of Insects?

    An exoskeleton cannot grow along with the organism; it must be shed to increase in size. So all arthropods periodically shed their exoskeleton during a process called ecdysis. The new cuticle is initially flexible - to allow the organism to expand to a new size - and then hardens.

  1. This assignment is about planning and designing practical experiment to carry out an investigation ...

    * The prepared plates were left in an oven for 2 days Then finally the area of no growth was measured Health and safety * Bunsen burner must be carefully used, especially when sterilising equipments, for example spreaders are made from glass so it must not left long on Bunsen

  2. Extended Experimental Investigation - Natural Antibiotics

    Albus Lime/Garlic [1:1] 11 S. Albus Lemon/Lime/Garlic [1:1:1] (again) 12 *The radius of sterilization refers to the average radius from the confetti stop that the antiseptic has effectively sterilized the agar so that it is impossible for the bacteria to survive. This graph documents the measures of effectiveness for each antibiotic tested.

  1. Investigating Seed Germination. Hypothesis If there is water, oxygen and a suitable ...

    - Normal amount of water; normal amount of oxygen; room temperature Set up 2) - Normal amount of water; normal amount of oxygen; 4�C (stored in the fridge) Set up 3) - Normal about of water; no oxygen; room temperature Set up 4)

  2. year 9 sycamore investigation

    Thus we dropped it at a height of 200cm. 3. What should the wind speed be when we drop the sycamore seed? In this situation we decide to play around with the anemometer. We discovered that no matter what you do ,the wind speed will never be the same unless it is zero.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work