• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Biology Practical Investigation to find the lowest concentration of Copper Sulphate solution that will denature egg albumen.

Extracts from this document...

Introduction

Biology Practical Investigation to find the lowest concentration of Copper Sulphate solution that will denature egg albumen Introduction Proteins are organic compounds of large molecular mass. They are not truly soluble in water; instead they form colloidal suspensions. Proteins are made up of amino acids.. There are three main types of bonding. The first is a disulphide bond. This occurs between cysteine molecules in the same amino acid chain (intrachain) or between molecules in different chains (interchain). This is the strongest and last bond to break. The second bond is an ionic bond. At a suitable pH, an interaction may occur between ionised amino and carboxylic groups. This forms an ionic bond. These ionic bonds are weak and can be broken in an aqueous solution by changing the pH of the medium surrounding the polypeptide. ...read more.

Middle

The arrangement of these bonds is the quaternary structure. When protein is denatured the specific shape formed by the four types of structures is broken down. Denaturation is a physical change in the shape of the protein molecule. This is due to the bonds, which normally hold the proteins specific three dimensional shape, being broken Aim of the Experiment To discover the lowest concentration of copper sulphate solution that will denature egg albumen. Prediction The copper sulphate ions will break down the tertiary structure causing the protein to denature. This will cause the egg albumen to turn white and opaque. The higher the concentration of the copper sulphate the faster and more opaque the albumen will turn, this is due to the increase in number of copper ions present in the solution. Apparatus 5 Test tubes Test tube holder Egg Albumen from a fresh egg 0.1Molar Copper Sulphate solution 5 Petri dishes Marker Pen 5ml Syringe ...read more.

Conclusion

This contains nothing other than pure water and no reaction will occur. This is called the control and all the results can be compared to it to determine whether a reaction has occurred. The fixed variables of this investigation are volumes of egg albumen used and the volume of copper sulphate solution. The independent variable is the concentration of copper sulphate. The dependant variable is whether the egg albumen has denatured or not. To ensure fair testing throughout the investigation the egg albumen used was from the same egg. The same volumes were used and measured using a syringe rather than a measuring cylinder, to ensure accuracy. The results obtained are qualitative, as there is nothing that can easily be measured. One way of obtaining quantitative results is to centrifuge the solutions after denaturation has occurred and this will show how much of the albumen has been denatured. Results Table Concentration of Copper Sulphate Solution Observations 0.01M 0.008M 0.006M 0.004M 0.002M 0. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Aqueous Chemistry section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Here's what a teacher thought of this essay

5 star(s)

*****
An excellent description of the structure of proteins.

The report is good thus far, consider how you could make the experiment more reliable (repeats) and how you can ensure that the results are accurate by exploring more variables that need to be controlled.

Also - you stated copper sulphate is an irritant if it makes contact with the
eyes, ensure you include goggles in the equipment list and safety precautions.

Marked by teacher Jon Borrell 08/01/2013

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Aqueous Chemistry essays

  1. Recrystallization - choose the most appropriate solvent to obtain a successful recrystallization of benzoic ...

    increase the surface area of the filter paper and hence faster the filtration process, so we can avoid the cooling process. Another way to minimize the problem, by using stemless funnel placed on top of the beaker containing few millimeters if the recrystallization solvent.

  2. Identification of an unknown organic compound

    We would assume a ketone is the functional group of the unknown organic compound. Apparatus needed would be same as the apparatus needed for the aldehyde test - Fehling's solution. If both of these tests were negative, we would then use the 2-4dinitrophenylhydrazine to see whether a carbonyl group was present.

  1. How much Iron (II) in 100 grams of Spinach Oleracea?

    Ammonium Sulphate (aq) is completely oxidised by the Potassium Manganate (VII) (aq)(7). Oxidation numbers provide an accurate way of following shifts in electron density during a reaction. Each atom in a molecule or ion is assigned an oxidation state at the beginning of the reaction and at the end of the reaction, and

  2. In this investigation I will get a number of unknown organic compounds to which ...

    OUTCOMES: A change from clear to a silver mirror precipitate on the side of the test tube indicates the presence of an aldehyde. No change indicates a ketone. EXPLANATION: Aldehydes are easily oxidised to acids. Ketones cannot be oxidised, as there is no place for the oxygen from the oxidising agent.

  1. An Experiment to Determine the Enthalpy Change for the Decomposition of Calcium Carbonate.

    using a measuring cylinder the meniscus may not have been exactly on the line which could have resulted in slightly higher or lower amount. * Not all the hydrochloric acid may have run into the polystyrene beaker this would have resulted in a slightly smaller volume.

  2. Indirect determination of enthalpy change of decomposition of sodium hydrogen carbonate by thermochemical measurement ...

    The weight came to 6.136g. I then added 2.0g of dry anhydrous sodium carbonate, ensuring that the weight of Na2CO3 was slightly over the stated 2.0g. This will help to minimise error, as some of the Na2CO3 might be left in the weighing bottle when we add it to the

  1. Investigating the effects of varying pH levels on the germination of cress seeds

    when diluting. Can be dangerous when mixed with hydrochloric acid; hydrogen chloride given off. Hydrochloric Acid: May cause burns. The vapour is very irritating to the respiratory system. Solutions of 1 M should be labelled 'irritant'. ETHICAL IMPLICATIONS From an ethical perspective, some may disagree with placing the cress seeds

  2. Indigestion Tablets Investigation

    Type and concentration of acid I will be using hydrochloric acid for all my experiments, as this is the same acid as that found in the stomach and therefore the reaction will be similar to that which could occur inside the body when somebody takes an indigestion tablet.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work