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GCSE: Green Plants as Organisms

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  1. Investigation using thin layer of chromatography to separate photosynthesis pigment

    As the solvent travels up slowly up the chromatography paper, the different components of the ink mixtures are separated into different colours spot due to the ink travelling at a different rate. Different factors affect the chromatography movement such as temperature; solvent; mixture and polarity. Temperature affects the movement of chromatography as the temperature of the solvent can dissolve better in a chemical that?s been transported at higher temperature. The solvent can affect the chromatography movement as a solvent with a strong interaction for a specific chemical will more easily overcome any affinity for the absorbent layer.

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