• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

An Investigation into the effect of substrate concentration (H2O2) on the rate of breakdown by catalase.

Extracts from this document...


An Investigation into the effect of substrate concentration (H2O2) on the rate of breakdown by catalase. 2H2O2 (l) O2 (g) + 2H2O (l) Hydrogen Peroxide Oxygen +Water Introduction: In this experiment I will be observing and comparing the rate of breakdown by the enzyme, 'Catalase' and Hydrogen Peroxide. The concentration of the Hydrogen Peroxide will be changed and, after the amount of oxygen is measured, I will be able to produce a table and graph of the changes in breakdown time. Aim: After I collect my results I will be able to plot a graph of the amount of oxygen produced over time, from which the rate of catalytic breakdown can de deduced. This will show how the rate of breakdown is affected by the substrate concentration and if there is any correlation between them, justifying my conclusion. Before the experiments begin, the method of conducting the experiment must be outlined meticulously. Method: In order to carry out my experiment, there must be a uniform method that I will use continuously during my experiment. I will be using an array of equipment that will be kept the same at all times. The experiment has a number of factors within it, which are easily adjustable, called variables. Therefore the controlled variables which must be controlled are: * Temperature- This can be controlled by using a water bath or ...read more.


The main Variables are:- * pH- This must be kept the same as it will affect the enzyme which is pH sensitive, and may denature or work better at certain pH's. * Time- I will have to keep the time of 20 seconds uniform and unchanged throughout the experiment by using a stopwatch correctly. * Temperature- This is relatively easy to keep controlled as the exothermic reaction is not too evident in the climate-controlled room. * The Concentration and volume of Catalase must be kept the same- by measuring the same amount of Yeast (5ml) each time. * The Volume of Hydrogen Peroxide must be the same- for each particular concentration the same amount of Distilled water and Hydrogen Peroxide must be used. These are the 'control variables' as they are being monitored, not changed. * The Concentration of the Substrate- the Hydrogen Peroxide will be changed to see how this affects my results. This is the 'Independent' Variable that I will be changing to affect the reaction rate and Volume of oxygen produced. * The volume of Oxygen produced after 35 seconds. This is the 'Dependant Variable' which 'depends' on the other variables, being unchanged to work Safety: First of all the procedure must be carried out in a safe and controlled way, where the risks are minimal and the appropriate precautions are taken to ensue maximum safety for more accurate results. ...read more.


This could gravely affect the results on my graph and appear to be an anomalously fast reaction. * In order to reduce the event of a gas-leakage, a firmly-placed rubber bung and tight-fitting rubber tubing must be applied otherwise the results could be badly compromised. I will also leave the syringe in the tubing so that no gas can escape from the apparatus. Prediction: "I predict that as the concentration of Hydrogen Peroxide decreases, the rate of reaction will decrease as well as the amount of Oxygen produced." This is because according to the collision theory when a substance has an increased concentration it has more available particles to collide with those of the other chemicals, and so by increasing the Concentration the rate of reaction should increase. When the concentration is low, there are less of the Hydrogen Peroxide particles available to react and so the reaction rate decreases, shown above by the few Hydrogen Peroxide particles that will react with the Yeast particles but will take much longer. The above diagram shows how when there are more Hydrogen Peroxide particles the reaction quickens, simply because of the fact that more particles are reacting simultaneously. Preliminary Results: Time (Secs) 5 10 15 20 25 30 35 Reading No. 1 15 26 37 46 56 61 66 2 21 29 29 39 46 52 56 3 15 28 42 48 60 70 78 ?? ?? ?? ?? - 1 - ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Living Things in their Environment section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Living Things in their Environment essays

  1. Marked by a teacher

    The aim of this investigation is to find out what effect pH has on ...

    4 star(s)

    H2O2, pH 4.4 solution, and potato etc * Grate the potato * Measure 3g of potato by putting the Petri dish on to the digital balance and setting it on 0g and then put the potato on the Petri dish until the scale reads 3g * Measure 20cm� of H2O2

  2. Investigating the Effect of Substrate Concentration on Catalase

    Preliminary Experiment/ Secondary Sources The preliminary experiment was to investigate the effect of pH on the enzyme catalase.12cm3 of H2O2 and 10cm3 of pH 2, 7 or 9 was added to a conical flask. A delivery tube was placed from the bottom of a burette in a trough of water into the conical flask.

  1. Osmosis Investigation

    When the potato tubors are placed in sucrose solution of concentration 0.25M, 4.24 - 4.00 x 100 4.00 = 6.00% is the percentage difference in length. When the potato tubors are placed in sucrose solution of concentration 0.5M, 3.78 - 4.00 x 100 4.00 = -5.50% is the percentage difference

  2. A investigation into the effect of inhibitor concentration on the enzyme catalase.

    It is used for removing Hydrogen Peroxide from cells. Hydrogen Peroxide is the poisonous by-product of metabolism. It speeds up the decomposition of hydrogen Peroxide into oxygen and water. It is able to do this because the shape of its active site matches the shape of Hydrogen Peroxide molecule.

  1. The effect of hydrogen peroxide on catalase if you change the temperature.

    With respect to I measured the height of froth in cm, but to be more precise I should have measured it in mm. To over come this I should use a ruler with mm readings. Also another problem that I observed on accuracy was that I didn�t allow the temperature to equilibrate to the right temperature.

  2. An Investigation into how the varying concentration of a substrate affects the rate of ...

    you increase the concentration of the substrate, the rate of reaction will be quicker because the more concentrated the molecules are, the more densely packed they are and so are more likely to collide and react. The rate of reaction will reach a plateau when there are higher concentrations because

  1. An investigation into the effect of a substrate concentration on the reaction rate of ...

    Hydrogen Peroxide Hydrogen peroxide (H2O2) is a very pale blue liquid which appears colorless in a dilute solution, slightly more viscous than water. It is a weak acid. It has strong oxidizing properties and is therefore a powerful bleaching agent that is mostly used for bleaching paper.

  2. Examine how the concentration of the substrate Hydrogen Peroxide (H2O2) affects the rate of ...

    Fill a beaker with about 4cm of the pre-made measured solution of Hydrogen Peroxide and using the graduated measuring cylinder, completely fill the 10 cm3 cylinder with the same solution. 3. Immerse the thistle funnel into the beaker with the steam below the surface of the Hydrogen Peroxide over the funnel stem.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work