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Introduction

Broad Bean Investigation Aim To investigate the ways in which temperature affects the period germination & growth of a broad bean over the duration of 21 days. I will keep 3 beakers in 3 different climates of temperatures and observe and note the alterations the bean will go through. These temperatures will be: * Room Temperature * In a chiller at a set temperature of 5�centigrade * And, in a water bath in the set temperature of 35� I will measure the shoots and roots of the beans and at the end of 3 weeks compare our results and see which temperature is ideal for a broad bean to grow in. Prediction I believe that in this investigation the beaker at room temperature will grow the best as I think this because the other two climates I believe are too extreme for the beans to grow in. The water bath I think may damage or kill the enzymes in the bean so there would be no growth. And I n the chiller the temperature would be too cold for any thing to grow in at all. I think that the cold would in fact slow down the growth of the broad bean So this is why I have come to the conclusion that it would be the best temperature at room temperature as you know in nature this is the temperature grows in. Variables Independent * The variable that will be changed throughout the investigation is the temperature. For beaker 1 I will have it at room temperature this I cannot control as this is only based on the outside temperature. * Beaker 2 is kept at the constant temperature of 35�but there is a problem with this which I will outline in Problems foreseen * And in the chiller the temperature will be kept at the constant 5�but again there is another problem. ...read more.

Middle

9. For temperature difference this does not matter as the beaker will only be taken out for short periods so no major temperature change will occur. Equipment 3x Beakers 1x Scissor 1x sheet of blotting paper 6x Broad Bean 1x Ruler 3x Thermometer 1x Water Bath 1x Supply of Water 1x Measuring Cylinder 1x Chiller Method How to prepare the beaker 1. Using a pair of scissors cut out three 20cm by 5cm strips of blotting paper. 2. Cut a slit in the blotting paper (see above) and put in the blotting paper around the beaker. 3. Insert the two beans into the pockets made earlier. 4. Fill up the beaker, using the measuring cylinder, up to 250 ml. 5. Put in a thermometer Method For setting up Water Bath Beaker 1. Fill up the water bath to about finger depth with water. 2. Switch Water Bath On 3. Set the water bath then to your chosen temperature e.g. 35� using the knob in the front. 4. And leave for 10-15 minutes in order for the water temperature to rise. How to Set Up For Beaker at Room Temperature 1. After preparing the beaker place the beaker in a place where sunlight can reach it.(Take care when placing making sure that the beans are not disturbed How to Set Up For Beaker in Water Bath 1. After preparing the beaker place the beaker in the Water Bath and secure with string so does not fall. As shown below. 2. Then place near the beaker at Room temperature ensuring that sunlight can reach it. How to Set Up For Beaker in Chiller 1. After preparing the beaker place the beaker take your beaker to the chiller and place at the back (where it is coldest) and close door. How to Measure the Shoots/Roots To measure the shoots and roots in a way that you do not cause any damage to them. ...read more.

Conclusion

For the bean in he chiller as I have said in Conclusion that the beans grew to about 4 mm. This is an anomalous result as it was believed that the cold would make the seeds not grow at all. But I believe that cold has instead just slowed down the growth of the beans. Also another solution may be that as there is no light in the chiller (see Problems Foreseen) the seed may have been searching for light as it is known that if a plant is in the dark the growth is fast in order for the plant to look for light so this happened but slower due to the cold. The blotting paper in the bakers we found became too soggy so this may have over watered the beans causing its growth to slow down. This may have affected the overall results so if I were to repeat this investigation I would ensure that every watering day the blotting paper is also replaced. I believe that there may have been some tampering with the beans as I could see that shoots looked a bit torn as if so this could have affected the overall outcome. If repeated again I would make sure that the beakers are taken somewhere where they will not be near anything which can harm them. I asked myself does timing effect anything as maybe if you give water too early the bean could die or too late and the bean is already dead. So I would make sure that timing has a part in the investigation as it could mean the bean dying or not. There was the problem of lack of sunlight and it did cause some problems to my overall results as seen in the results for the beaker in the chiller. We saw that the bean had grown which was not expected and meant that my results were not totally 100% accurate. Jahedur Rahman Broad Bean Investigation GCSE/GNVQ Coursework ...read more.

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