• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Catalase Investigation

Extracts from this document...


Catalase; Catalase is a heamoprotein with four sub units of equal size. It has a high affinity for its substrate, Hydrogen Peroxide, which is dramatically demonstrated by the violent reaction which takes place between the two. Catalase speeds up the decomposition of Hydrogen Peroxide, a toxic waste product of metabolic reactions. Catalase can be found in foods such as liver and potato and Catalase activity is reported in nearly all animal cells and organs and in aerobic micro-organisms. Specifically in cells it is located in the peroxisomes. These are vesicles produced by the Rough Endoplasmic Reticulum. Catalase reacts with Hydrogen Peroxide, removing it from cells by producing Hydrogen and oxygen as products, via the following equation; CATALASE 2H2O2 ( ) -------------------------------------------- 2H2O (I) + O2 (g) This is an example of an anabolic reaction. Since Catalase has reacted with Hydrogen Peroxide to break it down into smaller products. Catalase can be inhibited by ascorbate alone or copper ions - Cu2+ . This is also a factor affecting the rate of reaction. Therefore from my knowledge of enzymes in general and of Catalase more specifically l have formed my hypotheses, which l will test in my investigation. ...read more.


5. Here, various vegetables and fruit were used as the source of Catalase. These were much easier to cut exact cubes from, therefore controlling the surface areas and size much more precisely. Therefore from the choices available l chose set-up number 3 since this was the most accurate apparatus with the Catalase source or potato from number 5. I then went on to use the chosen piece of apparatus for my preliminary work. The results from this experiment are shown in the following table. Results from Preliminary Work; Gas given off (cm3) Time (min) 1 2 3 4 5 Concentration (M) 1 3.5 7 9 11.5 N/A 2 5 7 9 N/A N/A 4 3.5 126 179 2,112 14.5 APPARATUS LIST; Distilled water Glass rod Side-arm boiling tube Bung Glass beaker (500 cm3) Boss and clamp stand White tile Scalpel Callipers Thermometer Stop clock Goggles Cork borer (1c diameter) Gas syringe Potato (Catalase) Hydrogen Peroxide (4m) Syringes (10 ml, 20 ml) Ruler Graduated pipette and filler DIAGRAM OF APPARATUS METHOD; Prepare the various molarities of Hydrogen Peroxide, following the instructions as shown in the table below, to produce concentrations of 0.5M, 1.0M, 1.5M, 2.0M, 2.5M, 3.0M 3.5M, and 4.0M. ...read more.


This was varied in order to ascertain how the concentration of the substrate effects the action of the enzyme on its substrate. Concentration of Enzyme; controlled. If the concentration of the enzyme was not controlled the volume of Catalase each molarity of H2O2 was subjected to would be different and therefore the results would be unfair. Since the more volume of the enzyme present, the faster the rate of reaction since there are more active sites to act on the substrate and break it down into products quicker. This was controlled by cutting potato cylinder with a cork borer, then measuring out five 1cm3 cylinders with callipers, a ruler and a scalpel. This was, both the volume and surface area was controlled. Source of Catalase: controlled Depending on the variety of potato, the amount of Catalase present in each cell may vary. This would then have meant that although the size and surface area of the potato cylinder had been controlled the volume of the Catalase had not. By using the same breed and crop of potato it can be assumed that they have all been subjected to the same conditions and are, therefore, similar in the volume of Catalase they contain per cell. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Life Processes & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Life Processes & Cells essays

  1. For my coursework I will be performing an investigation into an experiment using hydrogen ...

    At the beginning and at the end of the experiment we will be monitoring the temperature of the room. A temperature change may have an affect on the experiment. 6. I may decide insulate the surface of the flask to keep in the heat, so that the results are more accurate.

  2. What influence does pH have on the enzyme Catalase?

    There is also an evident fair level of scatter shown by the wide error bars. This shows that that the data was not entirely reliable, perhaps due to method or human error, so mistakes could affect the reliability of the data.

  1. Investigating the Effect of Substrate Concentration on Catalase

    Hence, it would not be sensible to use pH2. The optimum pH for catalase is pH7 (http://en.wikipedia.org/wiki/Catalase) and my preliminary results show that it will produce results similar to that of my predicted results (as demonstrated in the background knowledge)

  2. Investigating the effects of temperature on an Enzyme Catalase.

    * I will make sure that all five bits of potato have the same surface area, because otherwise there will more catalase exposed, therefore creating a larger rate of reaction. * I am going to make sure there is no trapped air inside the syringe, before I do the experiment.

  1. Investigating Catalase.

    than it would be if there was just one big piece. The rate of reaction would increase, and more oxygen would be collected in the same amount of time than there would be if the surface area were smaller. * Type of vegetable - by changing the vegetable I would

  2. An Investigation into how the varying concentration of a substrate affects the rate of ...

    you increase the concentration of the substrate, the rate of reaction will be quicker because the more concentrated the molecules are, the more densely packed they are and so are more likely to collide and react. The rate of reaction will reach a plateau when there are higher concentrations because

  1. An investigation to find out how surface area affects the action of the enzyme ...

    Hydrogen peroxide. * Word equation: Hydrogen Peroxide Water + Oxygen * Symbol equation: 2 H2O2 2 H2O + O2 This reaction will help me to see how surface area affects the action of the enzyme, is speeded up by Catalase, a biological catalyst.

  2. An Investigation into the effect of surface area on the activity of catalase in ...

    This is not surprising considering the importance of tertiary structure (i.e. shape) in enzyme function and non-covalent forces, e.g., ionic interactions and hydrogen bonds, in determining that shape. 5 It is known that the enzyme catalase works best at pH 7, but is stable between pH 6 to 8, and

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work