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Chemistry Lab Chromatography

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Introduction

Title- Separation of dyes in screened methyl orange by paper chromatography. Introduction- chromatography is a technique used to separate the components of a mixture by making use of differences in solubility of the components. It can be used to separate such complex mixtures as the amino acids in blood or the various pigments in the flowers of a plant. In this experiment, the components of screened methyl orange which is a mixture of methyl orange (C14H14N3NaO3S) and xylene cyanol (C25H27N2NaO6S2) are separated through the use of water as a solvent. ...read more.

Middle

Some of the screened methyl orange was taken in the fine dropper and a spot of .5cm in diameter was made on the horizontal line. 3. 20cm3 of distilled water was poured into the 250cm3 beaker 4. The top of the chromatography paper was clamped to the retort stand and the lowered into the beaker ensuring that the spot of dye didn't touch the water. 5. The water was allowed time to rise then the chromatography paper was removed from the retort stand and the distance between the origin and the component was recorded. ...read more.

Conclusion

present on the chromatography paper. After calculating the retardation factor of each component it can be said that the blue component is more soluble that the yellow component in water. Sources of error-the dyes didn't separate completely by the time it reached high up on the chromatography paper so when recording values the highest point on the higher dye was recorded and the lowest point on the lower dye was recorded to avoid conflict. Conclusion-after completing the experiment the blue dye was found to be more soluble in water than the yellow dye. ?? ?? ?? ?? Varij Gosine ...read more.

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