Culturing Bacteria Making the Agar Gel and pouring it into the Petri dishes

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Culturing Bacteria

Making the Agar Gel and pouring it into the Petri dishes

Agar Gel ingredients:

* BOVRIL beef extract

* Peptone powder

* Bacteriological Agar

* Distilled Water

I will also need:

1. A pressure cooker

2. A stirrer

3. A beaker

Method:

First, sterilize the equipment by placing in the pressure cooker for 15 minutes. Mix these ingredients together and leave in a pressure cooker. Then, pour into Petri dish, and allow it to cool until the liquid agar solution solidifies.

Apparatus

1. Inoculating Loop - To apply the inoculum (the bacteria) to the surface of the agar gel in the Petri dish.

2. Bunsen burner - To flame the tops and necks of containers and to heat the tip of the inoculating loop in order to sterilize it.

3. Inoculum - The bacteria that will grow on the Petri dish.

4. Petri dish - Contains agar that will be the growth medium for the bacteria. Can be sealed by closing the lid and taping around it.

5. Sticky tape - to close the lid on the sides so that